黄精口服液对血管性痴呆大鼠学习记忆与海马突触可塑性的影响  

THE EFFECT OF POLYGONATUM ORAL LIQUID ON LEARNING AND MEMORY AND HIPPOCAMPAL SYNAPTIC PLASTICITY OF RATS WITH VASCULAR DEMENTIA

作　　者：赵小贞[1] 王玮[1] 康仲涵[1] 陈金水[2] 周琳瑛[3] 徐剑文[1] 

机构地区：[1]福建医科大学基础医学院人体解剖学与组织胚胎学系神经生物学研究中心 [2]福建医科大学附属第一医院中医科 [3]福建医科大学电镜室,福州350004

出　　处：《神经解剖学杂志》2005年第2期147-153,共7页Chinese Journal of Neuroanatomy

基　　金：福建省教育厅科研基金(JA00196)资助项目

摘　　要：应用Morris水迷宫、免疫组织化学法、透射电镜、图像分析和细胞形态计量学等技术,观察黄精口服液对血管性痴呆大鼠学习记忆能力和突触可塑性的影响。结果显示: (1)术后3. 5个月(即给药2. 5个月)痴呆+黄精口服液组大鼠平均逃避潜伏期较血管性痴呆组和痴呆+生理盐水组大鼠明显缩短(P<0. 05); (2)血管性痴呆组和痴呆+生理盐水组大鼠海马结构突触膜糖蛋白免疫反应产物校正灰度值比空白对照组明显降低;痴呆+黄精口服液组大鼠海马结构突触膜糖蛋白免疫反应产物校正灰度值高于血管性痴呆组和痴呆+生理盐水组大鼠(P<0. 05);大鼠平均逃避潜伏期与海马结构突触膜糖蛋白免疫反应产物校正灰度值呈负相关。(3)痴呆+黄精口服液组大鼠海马CA1区突触界面曲率增大、突触后致密物增厚、突触活性区增长(P<0.05)。这些结果表明:黄精口服液具有重塑突触结构与功能、改善血管性痴呆SD雌性大鼠学习记忆能力的作用。In order to observe the effect of polygonatum oral liquid on learning and memory and hippocampal synaptic plasticity of rats with vascular dementia (VD), the techniques of Morris water maze, immunohistochemistry technique, transmission electron microscope and morphological measurement were used in the present study. The results showed that:(1) The average escape latency of VD rats treated by polygonatum oral liquid was significantly shorter than the untreated VD rats. (2) The synaptophysin immunolabeling density of hippocampal formation decreased in VD rats in comparison with normal rats. The expression of synaptophysin in VD rats treated by polygonatum oral liquid was significantly higher than in those untreated. Furthermore, the average escape latency of rats was negatively correlated with the synaptophysin immunolabeling density in the hippocampal formation. (3) The curvature of synaptic interface, the thickness of postsynaptic density and the length of synaptic active zone were increased in rats treated by polygonatum oral liquid. The present results suggest that polygonatum oral liquid promots synaptic remodelling and improves learning and memory in rats with VD.

关 键 词：黄精口服液 血管性痴呆 大鼠 学习记忆功能 海马 突触结构 可塑性 

分 类 号：R285[医药卫生—中药学]