成年大鼠脑室下层细胞表达GFR-α_1的免疫组织化学研究  

IMMUNOHISTOCHEMICAL STUDY OF GFR-α_1 EXPRESSING CELLS IN THE SUBVENTRICULAR ZONE OF ADULT RAT

作　　者：刘方舟[1] 杨宁[1] 雷志年[1] 曾水林[1] 李涛[1] 王磊[1] 

机构地区：[1]东南大学神经生物学研究所,南京210009

出　　处：《神经解剖学杂志》2005年第2期199-202,共4页Chinese Journal of Neuroanatomy

基　　金：江苏省自然科学基金(BK2002056)资助项目

摘　　要：观察胶质细胞源性神经营养因子受体-α1 (GFR -α1 )在成年大鼠脑室下层(SVZ)细胞的表达,探讨胶质细胞源性神经营养因子(GDNF)对SVZ细胞的作用。成年大鼠SVZ组织冰冻切片,采用GFR -α1 结合5- 溴脱氧尿苷(BrdU)的免疫组织化学单标记与双标记方法,对成年大鼠SVZ进行观察。在成年大鼠SVZ可见GFR- α1 阳性细胞、BrdU阳性细胞和GFR- α1 /BrdU双标记细胞,且三种阳性细胞的分布趋势相似。结果提示GDNF可能参与调节成年哺乳动物脑内SVZ细胞的增殖、分化和迁移。In order to investigate the effect of glial cell line-derived neurotrophic factor (GDNF) in the subventricular zone (SVZ), the localization of GDNF family receptor-α_1 ( GFR-α_1 ) in cells of the SVZ of adult rat was examined with immunohistochemical staining for GFR-α_1 and/or 5-bromodeoxyuridine (BrdU). It was shown that GFR-α_1 and BrdU immunopositive cells and GFR-α_1/BrdU double-labeled cells were located in the SVZ. These results suggest that GDNF might have some important effects on the multiplication, differentiation and migration of cells in the SVZ of adult rat.

关 键 词：成年大鼠 脑室下层细胞 GFR-α1 免疫组织化学 胶质细胞源性神经营养因子 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]