根癌农杆菌介导库拉索芦荟遗传转化因素优化  

Factors optimization for genetic transformation in Aloe arborescens mediated by Agrobacterium tumefaciens

作　　者：潘颖南[1] 蒙平[1] 何铁光[1] 苏宾[1] 陈廷速[1] 李杨瑞[2] 

机构地区：[1]广西农业科学院生物技术研究所,广西南宁530007 [2]广西作物遗传改良生物技术重点开放实验室,广西南宁530007

出　　处：《广西植物》2005年第2期125-128,共4页Guihaia

摘　　要：以美国库拉索芦荟的横切薄片(transversethincelllayer,tTCL)作为转化外植体,初步研究了以根癌 农杆菌介导的多种因子对芦荟遗传转化的影响。结果表明:菌株EHA105比LBA4404及AGL1转化率高; 除了乙酰丁香酮(acetosyringone)外,菌液的预处理和重悬液的pH值也是影响转化的主要因子;菌液的预处 理和适合的蔗糖浓度对转化也有促进作用;感染时间为12～18min,共培养的温度和时间分别以25℃及5d 为佳。Transient expression of β-glucuronidase(Gus)gene were used to determine the optimized conditions for transformation of transverse thin cell layer(tTCL)of Aloe arborescens mediated by Agrobacterium tumefaciens. The results showed that EHA105 was more efficient than both LBA4404 and AGL1 in the genetic transformation. Besides acetosyringone(AS),pretreatment of the Agrobacterium liquid and the pH value of the suspension liquid(1/2MS)were also important in the genetic transformation. An appropriate concentration of sugar accelerated the transformation. The optimum infection time was 12 to 18 minutes. The best temperature and co-cultivation time were 25 ℃ and 5 days,respectively.

关 键 词：根癌农杆菌介导 遗传转化 美国库拉索芦荟 优化 乙酰丁香酮 cell 主要因子 蔗糖浓度 感染时间 预处理 外植体 转化率 pH值 共培养 菌液 

分 类 号：Q943.2[生物学—植物学] S682.29[农业科学—观赏园艺]