影响农杆菌介导玉米愈伤组织遗传转化因素的研究  

Study of influence factors on transformation of maize embryogenic callus by Agrobacterium tumefaciens

作　　者：农友业[1] 何勇强[2] 覃燕[1] Rachid Loukehaich 吴子恺[1] 

机构地区：[1]广西大学农学院,广西南宁530005 [2]广西大学生命科学技术学院,广西南宁530005

出　　处：《广西植物》2005年第2期142-144,i002,共4页Guihaia

基　　金：国家转基因植物研究与产业化专项项目(J00 003 03)

摘　　要：用农杆菌介导玉米愈伤组织的转化,其筛选的结果得到的抗性愈伤组织受玉米愈伤组织的继代时 间、浸染的农杆菌菌液浓度、共培养的温度以及其共培养时间等因素的影响。玉米愈伤继代后7～9d,农杆菌 浓度为OD600值0.3左右、共培养温度约22℃、培养时间3d时,抗性愈伤的获得率最高。Embryogenic callus of transformation of maize was generated by Agrobacterium tumefaciens. The result showed that resistant callus regenerated was influenced by the length of time of callus continuing culture,concentration of infecting A.tumefaciens,co-culture temperature and the length of co-culture time. The percentage of resistant callus regenerated was the highest when callus continuing culture time was 8～10 d,infecting A.tumefaciens concentration was OD (600)=0.3,the co-culture temperature was about 22 ℃ and the co-culture time was 3 d.

关 键 词：农杆菌介导 玉米 遗传转化 抗性愈伤组织 培养时间 共培养温度 继代时间 菌液浓度 

分 类 号：S511[农业科学—作物学] S513