光叶番荔枝中二萜类化合物对人肝癌细胞株SMMC-7721生长的抑制  

The inhibitory effect of diterpenoid compound isolated from annona glabra linn on the growth of human hepatocellular cancer SMMC-7721 cells lines.

作　　者：夏国豪[1] 章永红[2] 潘良熹[1] 

机构地区：[1]江苏省肿瘤医院内科,210009 [2]南京中医药大学

出　　处：《江苏医药》2005年第4期263-264,i002,共3页Jiangsu Medical Journal

基　　金：江苏省自然科学基金(BK2002201)

摘　　要：目的研究光叶番荔枝中二萜类化合物对人肝癌细胞株SMMC 7721 生长的抑制作用。方法以四唑盐比色试验(MTT)法检测二萜类化合物对SMMC 7721细胞生长的抑制率,用相差倒置显微镜观察细胞形态变化,用流式细胞术检测细胞周期、凋亡率及多药耐药基因1(MDR1)的表达水平。结果5~25μM的二萜类化合物对SMMC 7721细胞有较强的抑制作用,与浓度呈线性相关,并呈时间依赖性,72 h半数抑制浓度(IC50)为19 41μM ,20μM的药物作用于SMMC 7721 细胞48 h,流式细胞术检测细胞周期阻滞于G0 ~G1 期,细胞凋亡率为24 95%;在相差倒置显微镜下观察到细胞凋亡的特征形态改变,MDR1表达明显下调,且随药物浓度的增加MDR1 表达水平逐渐下降。结论光叶番荔枝中二萜类化合物对人肝癌细胞株SMMC 7721 有显著的抑制作用,可诱导癌细胞凋亡,明显下调MDR1的表达,具有逆转多药耐药性的作用。Objective To study the inhibitory effects of diterpenoid compound isolated from annona glabra linn on the growth of SMMC-7721 cells lines and its mechanism.Methods SMMC-7721 cells were treated with ~5μM ~25μM of diterpenoid compound.MTT assay was used to determine the cell growth rate. Flow cytometry(FCM) was used to examine cell cycle,cell apoptosis rate and MDR1 gene expression.Results In vitro studies, the diterpenoid compound could significantly inhibit the growth of SMMC-7721. After 48hours of treatment with 20μM diterpenoid compound, 'Sub-G_1'peaks were recorded by FCM, and the cell cycle was arrested in G_0/G_1 phase and the apoptotic rate was 24.95%. The expression level of MDR1 gene decreased after treateted with 5μM^25μM of the diterpenoid compound for 24 hours. Conclusion The diterpenoid compound isolated from annona glabra linn could inhibit the proliferation, induce apoptosis and downregulate MDR1 gene expression of ~human hepatocellular cancer SMMC-7721 cells lines.

关 键 词：二萜类化合物 人肝癌细胞株 光叶番荔枝 SMMC-7721细胞 流式细胞术检测 四唑盐比色试验 抑制作用 细胞形态变化 多药耐药基因 半数抑制浓度 细胞周期阻滞 显微镜下观察 MDR1 时间依赖性 细胞凋亡率 癌细胞凋亡 多药耐药性 

分 类 号：R282.71[医药卫生—中药学] R735.7[医药卫生—中医学]