脱细胞异种骨基质植入后受体外周血T淋巴细胞亚型的变化  

CHANGES OF SUBTYPE T LYMPHOCYTES IN BLOOD AFTER IMPLANTING WITH XENOGENEIC ACELLAR BONE MATRIX

作　　者：孙新君[1] 王正国[1] 朱佩芳[1] 周萍[1] 张宇[1] 刘大维[1] 张猛[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所

出　　处：《中国修复重建外科杂志》2005年第4期322-325,共4页Chinese Journal of Reparative and Reconstructive Surgery

摘　　要：目的　观察异种脱细胞去抗原骨基质植入后受体细胞免疫状态的变化。　方法　新西兰大耳白兔2 0只,随机分为4组,每组5只,制成股后肌袋模型,分别植入自体骨、异种脱细胞猪肋骨基质和酒精浸泡猪肋骨,设立假手术组作为对照。术后1、2、4和6周采集外周血,流式细胞仪检测外周血CD4 + 、CD8+ 和CD2 5 + T淋巴细胞的变化;分别于植入后2周和6周,将植入骨块连同周围组织取出,行组织学观察。　结果　同一时间点,外周血中CD4 +、CD8+ T淋巴细胞在酒精浸泡组显著增高,与其余组差异有统计学意义(P<0 .0 5 ) ,其余各组间差异无统计学意义(P>0 .0 5 ) ;CD2 5 + T淋巴细胞在酒精浸泡猪肋骨组2周后显著高于其它组,差异有统计学意义(P<0 .0 5 )。2周时局部组织中见炎性细胞浸润,以粒细胞为主;6周时自体骨及异种脱细胞骨基质组炎性细胞明显减少,代之以大量成纤维细胞及少量淋巴细胞,可见软骨岛形成。　结论　经脱细胞处理的异种骨基质植入后,近期对受体细胞免疫无明显影响。Objective To observe the changes of immune status in recipient after implanting with xenogeneic acellular bone matrix (ACBM). Methods Twenty rabbits were randomly divided into 4 groups. Autograft,ACMB and bone soaked in alcohol were implant into the 3 experimental groups separately, and No-treatment was done as control group. The CD4 +,CD8 +,CD25 + T lymphocytes in blood were detected by flow cytometer at 1, 2, 4 and 6 weeks after operation. After 2 and 6 weeks of implantation, the changes of bone and tissue were observed by histology. Results After 2-6 weeks, CD4 + and CD8 + T cells were significantly higher in the implanted group of bone soaked in alcohol than that in the other 3 groups( P <0.05) and there was no statistically significant difference in the other 3 groups( P >0 05). After 2 weeks, CD25 + T cells were significantly higher in the implanted group of bone soaked in alcohol than that in the other groups. In the 2nd week, there were inflammatory infiltration with a predominance of granulocytes. In the 6th week, there were many fibroblasts instead of granulocytes with a few lymphocytes and cartilage island formed in the implanted groups of autograft and ACMB. Conclusion ACBM implanting has low influence on cellular immunity in recipient.

关 键 词：T淋巴细胞亚型 骨基质 外周血 植入后 受体 CD8+T淋巴细胞 异种脱细胞 流式细胞仪检测 细胞免疫状态 酒精浸泡 炎性细胞浸润 CD4+ 组织学观察 成纤维细胞 脱细胞处理 统计学 大耳白兔 周围组织 局部组织 自体骨 新西兰 

分 类 号：R687[医药卫生—骨科学]