复方壳多糖真皮替代物体外抗菌作用的研究  

Study on Antibacterial and Antifungal Effect of Composite Chitosan Dermal Substitute in vitro

作　　者：雷霞[1] 伍津津[1] 朱堂友[1] 鲁元刚[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所皮肤科,重庆400042

出　　处：《中华皮肤科杂志》2005年第4期220-221,共2页Chinese Journal of Dermatology

基　　金：国家863课题(2001AA216041;2003AA205022);重庆市重点科技攻关项目(3897)

摘　　要：目的研究复方壳多糖真皮替代物对金黄色葡萄球菌、铜绿假单胞菌、大肠埃希菌、白念珠菌的体外抑菌作用。方法制备复方壳多糖真皮替代物和复方胶原凝胶真皮,分别为实验组和对照组,各取50μL滴加在涂满细菌或真菌的平板,每种菌实验组和对照组样本各6个,孵育24h后观察抑菌环。结果复方壳多糖真皮替代物对金黄色葡萄球菌、大肠埃希菌、铜绿假单胞菌具有抑菌作用,抑菌环均大于复方胶原凝胶真皮替代物,差异有统计学意义。两组对白念珠菌无抑菌作用。结论复方壳多糖真皮替代物是有体外抗细菌作用的组织工程产品。Objective To evaluate antibacterial and antifungal effect of the composite chitosan artificial skin in vitro. Methods The standard strains were used in the experiment, including Staphylococcus aureus (ATCC25923), Escherichia coli (ATCC25922), Pseudomonas aeruginosa (ATCC27853) and Candida albicans (ATCC10231). Twelve agar plates were prepared for each standard strain, which were equally divided into the trial and the control groups. In the trial groups, 50 μL composite chitosan dermal substitute was added to each prepared agar plate, two samples for each plate. In the control groups, composite collagen-gelatin dermal substitute was used. After the plates were incubated at 35 ℃ for 18 ~ 24 h, the antibacterial or antifungal rings of every sample were measured. Results The composite chitosan dermal substitute showed the antibacterial effect on Staphylococcus aureus (ATCC25923), Escherichia coli (ATCC25922), and Pseudomonas aeruginosa (ATCC27853) (P < 0.01, P < 0.05, and P < 0.001, respectively). Both composite chitosan dermal substitute and composite collagen-gelatin dermal substitute had no antifungal effect on Candida albicans (ATCC10231). Conclusion Composite chitosan artificial skin has the antibacterial effect in vitro, which may be used as a tissue-engineered substitute.

关 键 词：真皮替代物 复方壳多糖 体外抗菌作用 金黄色葡萄球菌 铜绿假单胞菌 复方胶原凝胶 大肠埃希菌 体外抑菌作用 组织工程产品 白念珠菌 抗细菌作用 对照组 实验组 抑菌环 统计学 

分 类 号：R318.0[医药卫生—生物医学工程]