两种变应原致小鼠变应性接触性皮炎的研究  

Dinitrofluorobenzene and Fluorescein Isothiocyanate Induced Mouse Model of Allergic Contact Dermatitis

作　　者：郭丽芳[1] 范卫新[1] 汤爱民[1] 

机构地区：[1]南京医科大学第一附属医院皮肤科,210029

出　　处：《中华皮肤科杂志》2005年第4期229-231,共3页Chinese Journal of Dermatology

摘　　要：目的建立两种变应性接触性皮炎(ACD)小鼠模型,并探讨其机制。方法分别以二硝基氟苯和荧光素-5-异硫氰酸盐作为致敏剂,以腹部致敏、耳部激发的方法建立两种ACD小鼠模型。激发前后测量耳廓厚度,计算耳肿胀度;H-E染色观察耳组织病理改变。用免疫组化法检测局部皮损中及用ELISA法检测淋巴结细胞分泌的Th1型/Th2型细胞因子水平。结果①成功建立两种ACD小鼠模型。②两种模型的局部皮损中细胞因子的表达有差异。二硝基氟苯组以白介素2表达为主;异硫氰酸荧光素组以白介素4表达为主。③二硝基氟苯组淋巴结细胞分泌干扰素酌的量是异硫氰酸荧光素组的5倍多;异硫氰酸荧光素组淋巴结细胞分泌白介素4的量是二硝基氟苯组的约2倍。结论二硝基氟苯诱导以Th1型细胞因子占优势的ACD,异硫氰酸荧光素诱导以Th2型细胞因子占优势的ACD。Objectives To establish allergic contact dermatitis (ACD) mouse models induced by dinitrofluorobenzene (DNFB) and fluorescein isothiocyanate (FITC), and investigate the pathogenesis of ACD. Methods Mice were sensitized by DNFB and FITC on the abdominal skin respectively, and challenged on the right ear, the left ear was treated with vehicle as the negative control. The thickness and degree of swelling of the ears were measured before and after the challenge. The histopathological changes were observed, and the expression levels of IL-2, IL-4, and IFN-γ in the ears and the lymph node cells (LNC) were detected with immunohistochemistry and ELISA, respectively. Results The thickness and swelling of right ears were significantly greater compared with the left ears (P < 0.05). IL-2 was mainly expressed in the DNFB treated ears, and IL-4 in the FITC treated ears. In the LNC, IFN-γ was mainly expressed in the DNFB treated mice, and IL-4 in the FITC treated mice. Conclusions The Th1 ACD is induced by DNFB, and the Th2 ACD is induced by FITC. CD4+ and CD8+ T cells may play the key role in the pathogenesis of Th1 and Th2 ACD, respectively.

关 键 词：变应性接触性皮炎 异硫氰酸荧光素 变应原 二硝基氟苯 ELISA法检测 TH1型细胞因子 Th2型细胞因子 免疫组化法检测 小鼠模型 细胞因子水平 细胞分泌 组织病理改变 白介素4 异硫氰酸盐 ACD 淋巴结 方法建立 耳肿胀度 白介素2 

分 类 号：R758.2[医药卫生—皮肤病学与性病学]