白念珠菌分泌型天冬氨酸蛋白酶基因在急性念珠菌性外阴阴道炎中的表达  

Expression of Candida albicans Secreted Aspartyl Proteinase in Acute Vaginal Candidiasis

作　　者：冯静[1] 涂亚庭[1] 林能兴[1] 冯爱平[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院皮肤科

出　　处：《中华皮肤科杂志》2005年第4期235-237,共3页Chinese Journal of Dermatology

摘　　要：目的了解白念珠菌分泌型天冬氨酸蛋白酶(Sap)基因在人类阴道白念珠菌感染时的表达情况。方法收集9例阴道念珠菌检测阴性及20例阴道念珠菌感染者的阴道分泌物,通过用特异引物系列进行RT-PCR反应来评价Sap1￣Sap6在人体阴道环境中的表达情况。结果Sap2和Sap5在阴道白念珠菌感染者中是最主要的表达基因;Sap3和Sap4在所有检查者中均被检测到;所有6种Sap基因在某些阴道念珠菌病患者中同时被检出。结论Sap基因可能与阴道念珠菌病的发病机制有关。Objective To analyze the expression of secreted aspartyl proteinases (Sap) in human vaginal infection in vivo. Methods Vaginal swabs were collected from 9 healthy volunteers and 20 patients with vaginal candidiasis, and the expression of Sap1-6 was evaluated by reverse-transcriptase polymerase chain reaction using specific primer sets. Results The Sap2 and Sap5 were the most common genes expressed in the patients with vaginal candidiasis. The expression Sap3 and Sap4 was detected in all subjects. All six Sap genes were simultaneously expressed in some patients with vaginal candidiasis. Conclusion The data shows that the Sap genes may be involved in the pathogenesis of vaginal candidiasis.

关 键 词：念珠菌性外阴阴道炎 蛋白酶基因 分泌型天冬氨酸蛋白酶 阴道念珠菌病 急性 白念珠菌感染 阴道白念珠菌 表达情况 阴道分泌物 PCR反应 表达基因 阴道环境 特异引物 发病机制 感染者 p基因 RT- 检查者 检测 Sa 

分 类 号：R711.31[医药卫生—妇产科学] R512.91[医药卫生—临床医学]