儿茶酚胺对豚鼠左心室流出道自律性电活动的影响  

Effects of cathcholamine on electrophysiological features of guinea-pig left ventricular outflow tract pacemaker cells

作　　者：赵兰平[1] 张晓云[1] 李建东[1] 陈彦静[1] 葛赋贵[1] 王德宝[1] 

机构地区：[1]河北北方学院生理教研室,张家口075000

出　　处：《陕西医学杂志》2005年第4期406-408,共3页Shaanxi Medical Journal

基　　金：河北省自然基金项目 (NO.3 0 0 3 79)

摘　　要：目的:探讨儿茶酚胺( CA)对豚鼠左心室流出道自律细胞电生理特性的影响。方法:用标准玻璃微电极细胞内记录技术,观测去甲肾上腺素( NE)、肾上腺素( E)和异丙肾上腺素( Iso)对左心室流出道自发慢反应电位的影响。观测指标:MDP、APA、Vmax、VDD、APD50 和APD90 时间以及RPF。结果:1 1 0 0 μmol/ L NE灌流后,RPF和VDD加快( P<0 .0 5 ) ,APD50 缩短( P<0 .0 1 ) ;2 1 0 0 μmol/ L E可使RPF( P<0 .0 1 )和VDD( P<0 .0 5 )加快,APA显著增大( P<0 .0 1 ) ,APD50 ( P<0 .0 1 )和APD90 ( P<0 .0 5 )缩短;3 1 0 0 μmol/ L Iso可使RPF和VDD显著加快( P<0 .0 1 ) ,MDP绝对值和APA显著增大,Vmax加速,APD50 和APD90 缩短。Objective: To explore the effects of norepinephrine(NE),epinephrine(E) and isoprenaline (Iso) on spontaneous slow action potentials(APs) of guinea-pig left ventricular outflow tract cells. Methods: By using standard intracellular microelectrode technique to record APs , NE,E and Iso were used to investigate the electrophysiological features of left ventricular outflow tract pacemaker cells. Electrophysiological parameters examined are:MDP, APA,Vmax,VDD,RPF, APD_ 50 and APD_ 90 .Results:①Perfusion with 100μmol/L NE led to a significant increase in VDD and RPF( P <0.05), a marked shortness in APD_ 50 ( P <0.01)in the pacemaker cells; ②Application of 100μmol/L E resulted in a significant increase in VDD( P <0.05), RPF( P <0.01) and APA( P <0.01) ,a notable shortness in APD_ 50 and APD_ 90 ( P <0.05);③100μmol/L Iso increased VDD, RPF, Vmax and APA significantly( P <0.01), decreased MDP notablely ( P <0.01), and shortened APD_ 50 and APD_ 90 markedly( P <0.01). Conclusion: CA could increase the autorhythmicity of left ventricular outflow tract.

关 键 词：左心室流出道 儿茶酚胺 自律性 豚鼠 电活动 mol/L 细胞内记录技术 APD90 去甲肾上腺素 异丙肾上腺素 玻璃微电极 电生理特性 慢反应电位 APA VDD 观测指标 VMAX RPF 缩短 Iso 绝对值 MDP 增大 

分 类 号：R331.3[医药卫生—人体生理学] R544.1[医药卫生—基础医学]