大鼠局灶性脑缺血后巨噬细胞浸润的研究  

A study of macrophage infiltration in focal cerebral ischemia of rat

作　　者：马琳[1] 何娅[1] 冯建军[1] 陈松盛[1] 王辉[1] 

机构地区：[1]西安交通大学第二医院老年病科,西安710004

出　　处：《陕西医学杂志》2005年第4期419-421,共3页Shaanxi Medical Journal

摘　　要：目的:探讨脑缺血后单核巨噬细胞浸润情况。方法:用免疫组织化学方法检测了30只大鼠大脑中动脉局部脑缺血1 2 h、2 4 h、3d、7d和1 0 d后损伤区ED2 阳性巨噬细胞浸润情况。结果:缺血1 2 h脑实质内可见ED2 阳性巨噬细胞,主要分布于坏死区周围;缺血2 4 h,阳性细胞较1 2 h有所增加,室管膜上有大量的阳性细胞聚集;缺血3d时阳性细胞数达高峰,主要存在于损伤区的周边区。缺血7d、1 0 d时染色中仅偶见阳性细胞的存在。统计学分析大鼠脑缺血1 2 h、2 4 h、3d时ED2 阳性巨噬细胞与正常对照组有非常显著性差异( P<0 .0 1 ) ,缺血3d组与1 2 h组相比差异也有显著意义( P<0 .0 5 )。结论:巨噬细胞参与了缺血性脑损伤的病理过程。Objective: To explore macrophage infiltration in focal cerebral ischemia of rat. Methods: The ED_2 positive macrophage was observed with the immunohistochemistry.The rats were subjected to middle cerebral artery occlusion(MCAO) for 12h,24h,3d,7d, and 10d. Results: The ED_2 positive macrophage was seen at hour 12 after MCAO around infarct area. At hour 24, there were more ED_2 positive macrophage than at hour 12 after MCAO , many macrophages appeared in ependyma. The cell number was the most at day 3 around infarct area. A few ED_2 positive macrophage were present in the cerebral tissue at day 7 and day 10. The cell number in the cerebral tissue at hour 12, hour 24 and day 3 Was significantly higher in comparison with controls( P <0.01),and there was also remarkable difference between day 3 and hour 12( P <0.05). Conclusion: Macrophage may be associated with the ischemic injury of brain , mainly in the early time of cerebral ischemia.

关 键 词：巨噬细胞浸润 缺血后 局灶性 免疫组织化学方法 缺血性脑损伤 阳性细胞 局部脑缺血 大脑中动脉 大鼠脑缺血 统计学分析 显著性差异 正常对照组 24h 脑实质内 细胞聚集 病理过程 病理损害 细胞数 

分 类 号：R692.31[医药卫生—泌尿科学] R339.146[医药卫生—外科学]