体外培养山羊早期胚胎基因表达的研究  

Preliminary study on the gene expression of preimplantation goat embryos produced in vitro

作　　者：李拥军[1,2,3] 敖红[2] 孙桂金[2,4] 赵有璋[1] 

机构地区：[1]甘肃农业大学动物科学与技术学院 [2]中国农业科学院畜牧研究所,北京100094 [3]扬州大学动物科学与技术学院,江苏扬州225009 [4]山东农业大学动物科学与技术学院,山东泰安271018

出　　处：《扬州大学学报（农业与生命科学版）》2005年第1期37-40,共4页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家自然科学基金资助项目(30270162)

摘　　要：用单胚构建的mRNA差异显示技术,对体外培养的山羊早期2、4、8-16细胞期胚胎的基因表达进行研究,并选择1条在8-16细胞期胚胎特异表达的条带进行分析。结果表明:该片段与牛NADH脱氢酶1a亚复合体4基因具有97%的同源性。该基因影响细胞内ATP的合成,可能与羊早期体外培养胚胎的正常发育有关。Single preimplantation embryo mRNA differential display was used to study the gene expression of 2-cell, 4-cell and 8-16-cell stage goat embryos cultured in vitro. A specific fragment expressed in 8-16-cell stage was selected for further analysis. The result suggested that this fragment displayed high homology (97%) to bovine NADH delydro-genase 1 alpha subcomplex-4. The protein encoded by this gene is essential for the production of ATP. This result indicated that the expression of ATP synthesis related genes at 8-16-cell stage may be required to maintain normal development of preimplantation goat embryos produced in vitro.

关 键 词：山羊 胚胎 单胚差异显示技术 基因 

分 类 号：S826.3[农业科学—畜牧学]