凉水、丰林国家自然保护区红松居群遗传多样性的RAPD分析  

Comparison of genetic diversity of Pinus koraiensis in two national nature reserves by RAPD marker

作　　者：吕建洲[1] 吴隆坤[1] 刘德利[1] 李岩[1] 裴赢[1] 张恒庆[1] 

机构地区：[1]辽宁师范大学生命科学学院,大连116029

出　　处：《植物研究》2005年第2期192-196,共5页Bulletin of Botanical Research

基　　金：国家自然科学基金(30170176)资助项目

摘　　要：采用了RAPD分子标记技术分析了凉水和丰林国家自然保护区红松(Pinuskoraiensis)居群的遗传多样性。10个随机引物检测到61个可重复的位点,多态位点比率为0. 693 5。Nei指数统计结果表明, 红松的遗传多样性凉水居群(0. 263 4)大于丰林居群(0. 260 7)。对比分析了这两个天然红松居群的遗传多样性变化,红松种内的遗传变异主要存在于居群内,居群间存在一定的遗传分化。With RAPD method, the paper studied genetic diversity and differentiation of two natural populations of Pinus koraiensis in Liangshui National Nature Reserve and Fenglin National Nature Reserve. Through the amplification of RAPD with 10 random primers, 61 repeatable loci were detected, in the amplification and percentage of polymophic loci was 0.693 5% . Nei’s index showed the levels of genetic diversity of Liangshui population( 0.263 4 ) was higher than Fenglin’( 0.260 7 ); genetic variation existed mainly within populations, there were little genetic differentiation between populations.

关 键 词：国家自然保护区 遗传多样性 RAPD分析 居群 红松 凉水 RAPD分子标记 技术分析 随机引物 多态位点 对比分析 遗传变异 遗传分化 可重复 

分 类 号：Q943[生物学—植物学] S759.992[农业科学—森林经理学]