超声提取—高效液相色谱法测定甘草中甘草酸含量  

Determination of the Glycyrrhizic acid content from Glycyrrhiza uralensis by HPLC

作　　者：付玉杰[1] 赵文灏[1] 侯春莲[1] 刘晓娜[1] 施晓光[1] 祖元刚[1] 

机构地区：[1]东北林业大学森林植物生态学教育部重点实验室,哈尔滨150040

出　　处：《植物研究》2005年第2期210-212,共3页Bulletin of Botanical Research

基　　金：哈尔滨市科技攻关计划项目(2004AA3CS172-2);东北林业大学科研启动基金

摘　　要：确定了制备甘草酸分析样品的超声波提取条件, 0. 3%稀氨水为溶剂,液固比50: 1,提取时间5h;确定了高效液相色谱法检测甘草酸含量的条件为ODS色谱柱(4. 6mm×25cm, 5μm),检测波长254nm,检测温度为室温,流动相CH3OH/3% HOAc(V/V)为75 /25,流速1mL/min,进样量10μL,平均加样回收率100. 20%,相对标准偏差为1. 77%。结果表明超声提取—高效液相色谱法是一种准确度高,速度快的测定甘草中甘草酸含量的检测方法。Extracted Glycyrrhizic acid (GA) from Glycyrrhiza uralensis Fisch by Ultrasonic extraction method, the conditions with higher GA were 0.3% ammonia solution as solvent, ratio of liquid to solid as 50:1(mL/g), extraction time five hours. The HPLC analysis conditions were as follows, chromatographic column ODS, detecting wavelength 254 nm , room temperature, mobile phase CH 3OH/ 3% HOAc(V/V)which ratio was 75/25, flowing rate 1 mL/min ,injected sample volume 10 μL . The result of percent recovery is 100.20% , the relative standard deviation is 1.77% . Ultrasonic extraction-HPLC is a fast detecting GA method with higher accuracy from Glycyrrhiza uralensis.

关 键 词：高效液相色谱法 草酸含量 超声提取 测定 相对标准偏差 CH3OH 提取条件 分析样品 提取时间 检测波长 检测方法 超声波 甘草酸 稀氨水 液固比 6mm 色谱柱 ODS 流动相 min 进样量 回收率 准确度 平均 

分 类 号：O657.72[理学—分析化学] TS272[理学—化学]