灵芝孢子几种破壁方法比较分析  

Comparing analysis on several wall-cracking methods of Ganoderma lucidum spore

作　　者：薛艳华[1] 史权[1] 庞海河[1] 韩梅[1] 杨蕾[1] 

机构地区：[1]东北林业大学森林植物生态学教育部重点实验室

出　　处：《植物研究》2005年第2期216-218,共3页Bulletin of Botanical Research

摘　　要：采用超临界破壁法、匀浆破壁法和超声破壁法对灵芝孢子进行了破壁试验研究,研究显示,采用超临界高压静止破壁法可显著提高灵芝孢子的破壁率。匀浆破壁法和超声破壁法虽灵芝孢子破碎率较低,显微镜观察大部分孢子外形完整,但细胞内容物已几乎消失,达到提取孢子内容物的目的。Several methods on the wall-cracking of Ganoderma lucidum spore were carried on, mainly wall-cracking methods included supercritical wall-breaking, homogenate wall-cracking and ultrasonic wall-breaking. The results showed that adopting supercritical high-pressure steady state wall-cracking method could particularly improve the wall-crushing rate of Ganoderma lucidum spore. Although the wall-cracking rate of homogenate wall-cracking and ultrasonic wall-cracking was lower and most of the spores of Ganoderma lucidum were integrate in the microscope,the contents were nearly disappeared, attach to the purpose extracting effective constituentof spore inner.

关 键 词：灵芝孢子 比较分析 破壁方法 显微镜观察 试验研究 超临界 内容物 破壁率 破碎率 超声 匀浆 

分 类 号：S567.31[农业科学—中草药栽培] S896.4[农业科学—作物学]