脐血CD34^+CD38^-早期造血祖细胞的体外增殖和凋亡  

Proliferation and Apoptosis in vitro of Umbilical Cord Blood CD34^+ CD38 ^- Hematopoietic Early Progenitor Cells

作　　者：田虹[1] 黄士昂[2] 龚非力[1] 郑金娥[2] 贺艳丽[2] 杨晶[2] 陈忠[1] 

机构地区：[1]武汉华中科技大学同济医学院基础医学院医学生物系,武汉430030 [2]武汉华中科技大学同济医学院附属协和医院干细胞中心,武汉430022

出　　处：《中国实验血液学杂志》2005年第2期229-234,共6页Journal of Experimental Hematology

基　　金：国家杰出青年基金资助(30225038)国家973基金部分资助(001CB510103)

摘　　要：为了从适龄产妇脐血中分离出CD34+CD38-细胞,在体外较长时间培养后观察分析CD34+CD38-细胞分 裂增殖、凋亡以及干细胞因子对CD34+CD38-细胞增殖的影响,用流式细胞仪从10例健康产妇脐血中分选出 CD34+和CD38-标记的脐血原始细胞,在添加IL-3、IL-6、GM-CSF、EPO、IGF-1和SCF 6种混合因子的干细胞培养基 中培养6个月,观察细胞并绘制生长曲线,用单细胞凝胶电泳检测干细胞因子对CD34+CD38-细胞生长的影响和 用流式细胞仪检测CD34+ CD38-细胞凋亡情况。结果表明:脐血CD34+CD38-细胞可在体外较长时间培养增殖, 无异常或过度的细胞凋亡发生。结论:通过控制培养条件,脐血CD34+CD38-早期造血祖细胞可在体外较长时间 培养增殖,以作为大量脐血原始细胞移植的细胞来源。To cultivate CD34 + CD38 cells isolated from umbilical cord blood of healthy puerperal women over a longer-period of time for observation of cell division, proliferation, apoptosis, and effects of stem cell factor on the growth of CD34 + CD38 - cells, with flow cytometry, CD34 + CD38 - cells were isolated from umbilical cord blood of 10 healthy puerperal women and cultivated in stem cell media with supplement of IL-3, IL-6, GM-CSF, EPO, IGF-1 and SCF 6 kinds cell growth stimulating factors for six months. The cell growth curves were established. The effects of stem cell factor on the growth of CD34 + CD38- cells and cell apoptosis were investigated with the single cell gel electrophoresis technique and flow cytometry method, respectively. The results showed that CD34 + CD38 - cells isolated from umbilical cord blood were capable of proliferating after being cultivated in vitro over a longer-period of time with no evidence of the presence of excessive apoptosis. In conclusion, under appropriate culture conditions, CD34 + CD38 - hematopoietic early progenitor cells from umbilical cord blood can serve as a resource providing a large amount of primitive cells for transplantation therapy after a longer period of cultivation and proliferation in vitro.

关 键 词：脐血 CD34^+ CD38^-细胞 体外增殖 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]