用α-半乳糖苷酶制备可供输注的人通用型O型红细胞  

Preparation of Transfusable Human Universal Red Blood Cell with Recombinant α-Galactosidase

作　　者：宫锋[1] 吕秋霜[2] 由英[1] 高红伟[1] 鲍国强[1] 高新[1] 李素波[1] 李立立[1] 王颖丽[1] 田曙光[1] 张志欣[2] 张评[2] 章扬培[1] 

机构地区：[1]军事医学科学院野战输血研究所,北京100850 [2]北京市红十字血液中心,北京100088

出　　处：《中国实验血液学杂志》2005年第2期313-316,共4页Journal of Experimental Hematology

基　　金：国家"973"(2002CB713804);全军十五重点课题(01Z029)资助

摘　　要：研究的目的是将人群中28％的B型血改造成通用O型血,提高O型血的储存和使用比例,以缓解战争、 恐怖袭击、突发事件等紧急状态下对O型血的大量需求。用α-1.3半孔糖苷酶作为B→O血型改造的工具酶,摸索 改造一个使用单位B型血红细胞(100 ml)的最佳条件。结果实现了在密闭、无菌条件下使用50 U／ml工具酶,pH 5.6,26℃ 2小时,进行B→O的血型改造。结论:改造后的通用型O型血红细胞符合生物制品检定规程的要求, 并可在4℃存放21天。In order to meet the demand for safe transfusion in special conditions and to utilize the donated blood supply efficiently, technology has been developed to convert erythrocytes from type A, B, or AB to 'universal donor' blood. Conversion of blood type B to O was performed by means of recombinant a-galactosidase digestion. The results showed that blood type B to O was converted successfully, 1 transfusion unit of red cells of group B ( 100 ml totally) could converted to universal blood cells in the optimal conditions including pH 5. 6, 26℃, 2 hours, obturation and sterilization. It is concluded that the universal red blood cells converted from group B to group O are conformed to demand of identification rules of biological products, no harmful effects of a-galactosidase on cell structure and function are observed. The converted red cells can stored in 4℃ for 21 days.

关 键 词：输血 O型红细胞 α-1.3半乳糖苷酶 血型改造 B→O血型改造 

分 类 号：R457.11[医药卫生—治疗学]