B型全血加入不同剂量0型全血后游离血红蛋白变化  

Change of Free Hemoglobin Content after Mixing Type B Whole Blood with Different Amounts of Type O Whole Blood

作　　者：肖瑞卿[1] 赵树铭[1] 曾杰[1] 许聃[1] 陈英[1] 吴建君[1] 胡建[1] 许汝福[2] 刘景汉[3] 

机构地区：[1]第三军医大学附属西南医院输血科,重庆400038 [2]第三军医大学预防医学系,重庆400038 [3]中国人民解放军总医院输血科,北京100039

出　　处：《中国实验血液学杂志》2005年第2期323-325,共3页Journal of Experimental Hematology

摘　　要：为了解B型全血加入不同剂量O型全血后在37℃与室温条件下游离血红蛋白的变化,随机抽取4℃保 存24小时B型全血两人份,分别分装为60 ml,按不同比例加入O型全血9、12、15、18 ml置入1 00ml塑料血袋内 混匀后,分别放置在37℃孵箱和室温条件下,间隔15分钟摇动1次。分别在1、2、4、8和12小时留取标本做游离血 红蛋白检查。结果表明:B型全血加入不同剂量O型全血,在12小时内游离血红蛋白量的变化无显著性差异。随 着保存时间的延长不管是室温还是37℃条件下,1小时以后均有显著性差异,一袋B型全血在室温与37℃条件下 放置1-8小时无明显差别(P>0.05),到12小时P<0.001。另一袋在室温与37℃条件下放置1小时P>0.05,2 -8小时P<0.001,有明显差别。结论:在B型全血中加入O型全血,放置12小时不会引起游离血红蛋白升高。 在室温与37℃存放8小时后接近和超过存放2天的水平170.4 mg／L,这提示血液采集后应尽快置入2-4℃冷藏, 减少红细胞的分解代谢、衰老裂解而释放更多的FHb及其它代谢物质时机体的损害。To investigate the changes of free hemoglobin (FHb) content after mixing type B whole blood with different amounts of type O whole blood at room temperature and at 37℃, two lots of type B whole blood stored at 4℃ for 24 hours were randomly taken as recipient blood, and were packed as 60 ml respectively. Type O blood was taken as donor blood. 60 ml type B whole bloods were mixed with different amounts of type O whole blood, i. e. with 9, 12, 15 and 18 ml. The mixed blood was packed into 100 ml plastic blood bags and stored at 37℃ or room temperature, snaked once every 15 minutes. Free hemoglobin content was determined for the harvested samples at 1, 2, 4, 8 and 12 hours after store. The results showed that there was no significant elevation of FHb within 12 hours after mixing B whole blood with different amounts of type O whole blood. In another lot, there was obvious difference in FHb after 1 hour store along with the prolongation of store at either room temperature or 37℃. In one lot, there was no difference of FHb (P > 0.05) during 1 - 8 hours of store at room temperature or 37℃, but significant difference at 12 hours of store (P < 0. 001). In another lot, there was no difference of FHb (P > 0.05) within 1 hour of store at room temperature and at 37℃, but significant difference during 2 ~ 8 hours of store (P <0.001). It is concluded that the FHb would not change significantly within 12 hours after type B bood was mixed with 1 200 ml of type O whole blood, but when the mixed blood was placed at room temperature or at 37℃ for 8 hours, the FHb content approaches, even exceeds 170.4 mg/L which was observed in the blood stored for 2 days. It suggests that freshly collected blood must be put into refrigerator of 2 ~4℃ for storing as soon as possible, so as to decrease the catabolism of erythrocyte and the releasing of FHb and other metabolites which are deleterious to the recipients.

关 键 词：B型全血 O型全血 游离血红蛋白 抗体效价 

分 类 号：R457.11[医药卫生—治疗学] R331.141[医药卫生—临床医学]