东亚飞蝗感染绿僵菌后的组织病理变化  

The histopathological changes of Locusta migratoria manilensis after infection with Metarhizium anisopliae

作　　者：黄冬如[1] 雷仲仁[1] 王国平[2] 问锦曾[1] 

机构地区：[1]中国农业科学院植物保护研究所,北京100094 [2]湖南农业大学植物保护学院,长沙410128

出　　处：《昆虫知识》2005年第2期183-185,F002,共4页Entomological Knowledge

基　　金：科技部"十五"攻关项目 (2 0 0 4BA5 0 9B18);公益研究专项资助 (340 0 7)

摘　　要：将人工培养的绿僵菌分生孢子配制成浓度为 1× 1 0 8分生孢子 mL的孢子油剂 ,涂抹在供试东亚飞蝗Locustamigratoriamanilensis(Meyen)幼虫的腹侧部。通过组织切片研究表明 ,绿僵菌主要是从东亚飞蝗体表侵入的 ,72h后可见体腔内有菌丝和组织病变 ,侵入体内的菌丝在血腔中不断增殖 ,使得脂肪体、肌肉组织、马氏管和消化道发生病变解体。 96h后 ,肠壁细胞结构开始疏松、内膜解体。 1 2 0h后 ,多数幼虫死亡 ,消化道内外布满菌丝。The pathological changes of Locusta migratoria manilensis infected by Metarhizium anisopliae were observed using tissue slice. The 5th larval abdominal integument was the major invasion route of Metarhizium anisopliae.After 72 h,hyphae were be observe in the coelum and began to repoduce in the hemocoele.And then,the epiploon,muscle tissue,malpighian tubules and digestive tract began to undergo pathological changes.The cells in the midgut tissues were loosened and inner membrane were disintegrated.Most of the larvae died on the fifth day,and the digestive tract was covered up with hyphae outside and inside.

关 键 词：东亚飞蝗 组织病理变化 感染 分生孢子 人工培养 组织切片 组织病变 肌肉组织 细胞结构 绿僵菌 消化道 菌丝 脂肪体 马氏管 幼虫 侵入 解体 体表 增殖 

分 类 号：S476.12[农业科学—农业昆虫与害虫防治]