蚧虫基因组DNA不同提取方法的比较  

Comparative study of methods for isolation of genomic DNA of scale insects

作　　者：石晶[1] 谢映平[1] 薛皎亮[1] 姚国亲[1] 

机构地区：[1]山西大学生命科学与技术学院,太原030006

出　　处：《昆虫知识》2005年第2期207-211,共5页Entomological Knowledge

基　　金：国家自然科学基金资助项目 (30 2 710 87);山西省自然科学基金项目 (2 0 0 310 6 5 )

摘　　要：实验以日本龟蜡蚧CeroplastesjaponicusGreen,白蜡绵粉蚧PhenacoccusfraxinusTang ,朝鲜球蚧DidesmococcuskoreanusBorchseniush和瘤大球坚蚧EulecaniumgiganteaShinji等 4种蚧虫为材料 ,分别用十二烷基硫酸钠 (SDS)法、十六烷基三乙基溴化铵 (CTAB)法、醋酸钾 (KAc)法和氯化钠 (NaCl)法等 4种方法 ,对单只蚧虫进行基因组DNA提取 ,用 0 8%琼脂糖凝胶电泳检测所提DNA。结果表明 ,4种方法都可以提取到基因组DNA ,但是比较而言 ,CTAB法和NaCl法所提取的DNA质量明显优于SDS法和KAc法 ,并适用于PCR。因此认为 ,CTAB法和NaCl法是实验室提取单只蚧虫基因组DNA更有效而实用的方法。Genomic DNA were extracted from the scale insect species: Ceroplastes japonicus Green, Phenacoccus fraxinus Tang, Didesmococcus koreanus Borchseniush, and Eulecanium gigantea Shinji, by using the sodium dodecyl sulfate(SDS) method, the cetyltriethylammonium bromide(CTAB) method, the sodium chloride(NaCl) method, and the acetic acid potassium (KAc) method respectively. The each DNA sample was extracted from the whole body of a single scale insect and was tested by 0.8% agarose gel following electrophoresis. The results suggest that, to some degree, the four methods were useful in extraction of genomic DNA of scale insects. But comparatively, the quality of DNA samples isolated by using the CTAB method and the NaCl method was obviously better and more suitable for PCR than that by using the other two methods. Therefore, the CTAB method and the NaCl method were more effective and practical method in the genomic DNA extraction of a single scale insect in laboratory.

关 键 词：蚧虫 提取方法 基因组DNA提取 十二烷基硫酸钠 CTAB法 NaCl 日本龟蜡蚧 Green 白蜡绵粉蚧 瘤大球坚蚧 琼脂糖凝胶 十六烷基 电泳检测 SDS法 溴化铵 三乙基 氯化钠 醋酸钾 AC法 PCR 实验室 

分 类 号：Q966[生物学—昆虫学]