系统性红斑狼疮患者血清B细胞激活因子水平的检测  

Measurement of serum B lymphocyte stimulator levels in patients with systemic lupus erythematosus

作　　者：周琳[1] 屠小卿[1] 朱烨[1] 陆慧琦[1] 许臻[2] 范列英[1] 侯晓菁[1] 孔宪涛[1] 仲人前[1] 

机构地区：[1]第二军医大学长征医院实验诊断科全军临床免疫中心,上海200003 [2]第二军医大学长征医院风湿内科,上海200003

出　　处：《中华风湿病学杂志》2005年第4期222-224,共3页Chinese Journal of Rheumatology

基　　金：国家自然科学基金资助项目(30400409)

摘　　要：目的建立检测血清B细胞激活因子(B lym phocyte stim ulator,BlyS)水平的酶联免疫吸附试验(ELISA)方法,并检测系统性红斑狼疮(SLE)患者的血清BlyS水平,初步探讨BlyS与SLE发病机制的关系。方法以商品化的抗人BlyS单克隆抗体、生物素化抗人BlyS多克隆抗体,采用双抗夹心法建立检测BlyS血清水平ELISA方法。对59例SLE确诊患者和40名正常健康献血者的血清BlyS水平、免疫球蛋白(IgG、IgA、IgM)浓度、自身抗体[抗双链DNA抗体(抗dsDNA抗体)]滴度进行检测,比较SLE患者的血清BlyS水平与免疫球蛋白浓度、自身抗体滴度的相关性。结果59例SLE患者的血清BlyS水平为(8.4±1.6)ng/m l,40名正常人的血清BlyS水平为(3.2±0.9)ng/m l,SLE组的BlyS血清水平明显高于正常人组(P<0.01);且伴随着血清BlyS水平的升高,SLE患者的血清IgG浓度明显升高(r=0.442,P=0.0005);SLE患者的BlyS血清水平与抗dsD NA抗体的滴度也有明显的相关性(r=0.850,P<0.01)。结论本研究所建立的BlyS血清水平ELISA检测方法简便、可靠、准确,为后续研究BlyS与其他自身免疫性疾病的关系奠定了基础。SLE患者的血清BlyS含量是明显升高的,并伴随着血清IgG浓度、抗dsD NA抗体滴度的升高;提示Blys异常表达可能是SLE发病的重要环节,其对自身反应性B细胞的活化。Objective To establish an immune enzyme assay for detecting the serum levels of B-lymphocyte stimulator (BlyS) and to investigate the relationship between serum BlyS levels and systemic lupus erythematosus (SLE). Methods Anti-human BlyS monoclonal antibody and biotinylated anti-human BlyS polyclonal antibody were used to detect serum BlyS levels by ELISA. Serum samples from 59 patients with SLE were assayed for BlyS levels, quantitative immunoglobulins (IgG, IgA, IgM), and autoantibody (anti-double-stranded DNA antibody) levels were also measured, and were compared with samples from 40 healthy control subjects. Results The median level of serum BlyS was (8.4±1.6) ng/ml in SLE patients and (3.2±0.9) ng/ml in healthy controls (P<0.01). The higher level of BlyS was associated with higher level of IgG (r=0.442, P=0.0005). There was a strong correlation between serum BlyS levels and anti-double-stranded DNA antibody titers among SLE patients. Conclusion In this study, serum BlyS levels detected by enzyme-linked immunosorbent assay (ELISA) is reliable, simple and precise and may be used as a valuable marker for investigation of the relationship between BlyS and other autoimmune diseases. In human SLE, the serum BlyS levels are significantly elevated and correlated with the level of IgG and autoantibidies. Thus, dysregulation of BlyS is common in patients with SLE. BlyS may play an important role in activating specific autoreactive B cells and modulating the level of autoantibodies production.

关 键 词：B细胞激活因子 系统性红斑狼疮患者 酶联免疫吸附试验(ELISA) 抗DSDNA抗体 lymphocyte SLE患者 抗双链DNA抗体 BLYS ELISA方法 自身免疫性疾病 特异性自身抗体 血清IGG 血清水平 自身抗体滴度 单克隆抗体 多克隆抗体 健康献血者 

分 类 号：R593.241[医药卫生—内科学]