系统性红斑狼疮患者T细胞上Fas受体分子与疾病活动指数的相关性研究  

The correlation between quantitative expression of Fas receptor molecules on CD3^+ T lymphocyte and disease activity of systemic lupus erythematosus

作　　者：李富荣 肖学吕 叶志中[3] 齐辉 任莉莉 张丽君[3] 王新根 

机构地区：[1]深圳市人民医院(暨南大学医学院第二附属医院)临床医学研究中心,518020 [2]深圳市人民医院(暨南大学医学院第二附属医院)风湿免疫科,518020 [3]广东医学院深圳市风湿病研究所

出　　处：《中华风湿病学杂志》2005年第4期233-235,共3页Chinese Journal of Rheumatology

基　　金：广东省自然科学基金资助项目(021339)

摘　　要：目的通过定量流式细胞术检测系统性红斑狼疮(SLE)患者T淋巴细胞表面Fas受体的分子数,探讨SLE的发病机制及与疾病活动指数的相关性。方法对36例活动期SLE患者和18名正常人群采集外周血,采用Fas定量流式细胞试剂盒荧光染色,通过流式细胞仪对T淋巴细胞上表达Fas受体分子数和凋亡率进行检测。结果活动期SLE患者T细胞表面表达Fas受体分子数和凋亡率较正常人群明显上调(P<0.01)。活动期SLE患者T细胞表面Fas受体分子数与T细胞凋亡率和SLEDA I之间,具有明显的正相关(P<0.01),抗dsDN A抗体阳性患者组T细胞表面Fas受体分子数较抗dsDN A抗体阴性组明显增高,差异具有统计学意义(P<0.05)。结论SLE患者T淋巴细胞表面Fas受体分子数上调,由Fas介导的T淋巴细胞凋亡加快,刺激机体产生抗dsDNA等多种自身抗体,可能是引起SLE免疫功能紊乱的主要原因。SLE患者T淋巴细胞表面Fas受体分子数与SLE的活动性呈正相关,是一较好的评价SLE疾病活动性的指标。Objective To study the expression of surface Fas receptor molecules on CD3+ T lymphocytes, and to investigate the relationship with pathogenesis and activity of systemic lupus erythematosus (SLE). Methods Dual flow cytometry was used to quantitatively measure the expression of Fas receptor molecules and apoptosis on CD3+ T lymphocytes from 36 active patients with systemic lupus erythematosus and 18 healthy individuals. Results The expression of Fas receptor molecules and apoptosis on CD3+ T lymphocytes of SLE patients were significantly higher than that of healthy group (P<0.01). There was positive correlation between Fas receptor molecule, percentage of CD3+ T lymphocytes and activity index of SLE (P<0.01). On the other hand ,Fas receptor molecule expression on CD3+ T lymphocytes from the patients with positivity anti-dsDNA antibody was high, compared with that of patients with negative anti-dsDNA antibody (P<0.05). Conclusion The results suggest that the expression of Fas receptor molecules on CD3+ T lymphocytes from SLE patients is relatively high. Increased expression of Fas receptor molecules on CD3+ T lymphocytes accelerates the apoptosis of lymphocytes and stimulates the production of antibodies. It is an indicator for disease activity evaluation in SLE patients.

关 键 词：疾病活动指数 受体分子 系统性红斑狼疮患者 相关性研究 T细胞 SLE患者 抗dsDNA抗体 流式细胞术检测 T淋巴细胞凋亡 SLEDAI 细胞表面表达 多种自身抗体 免疫功能紊乱 正常人群 FAS受体 细胞凋亡率 流式细胞仪 FAS介导 疾病活动性 

分 类 号：R593.241[医药卫生—内科学] R965.1[医药卫生—临床医学]