过敏性紫癜患儿淋巴细胞活化、分泌及雷公藤对其作用  

Activation and secretion of peripheral blood lymphocytes in children with Henoch-Schonlein purpura and the therapeutic effects of triptolide on them

作　　者：夏志强[1] 卢思广[1] 郭维[1] 

机构地区：[1]徐州医学院儿科研究室,江苏徐州221002

出　　处：《徐州医学院学报》2005年第2期124-126,共3页Acta Academiae Medicinae Xuzhou

摘　　要：目的　探讨过敏性紫癜(HSP)患儿外周血淋巴细胞(PBLs)活化、分泌状态的变化以及雷公藤内酯醇(TP)对其作用。方法　常规分离PBLs,以流式细胞仪检测CD3、CD2 5抗原表达率,以EILSA法检测白细胞介素-4 (IL - 4 )分泌水平。结果　与健康儿童比较,HSP患儿PBLs培养0、4 8hCD+ C D+ 2 5细胞百分率及IL - 4分泌水平均明显升高(P <0 .0 5 ) ;应用TP后,培养4 8hHSP患儿PBLsCD3(+)CD2 5 (+)细胞百分率及IL - 4分泌水平均明显降低(P <0 .0 5 )。结论　HSP患儿PBLs活化及分泌水平异常增高,可能参与HSP的发病机制;TP抑制PBLs活化及分泌,可能是雷公藤对HSP作用机制之一。Objective To explore the activation and secretion of peripheral blood lymphocytes (PBLs) cultured from children with Henoch-Schonlein purpura (HSP) and the effects of triptolide(TP) on them.Methods PBLs were cultured from 23 children with HSP and an equal number of healthy controls to test the effects of TP on them. The expression of CD3 and CD25 was assessed by flow cytometry, the IL-4 content in the supernatant of cultured PBLs was determined by ELISA.Results The percentage of CD3(+)CD25(+) cells and the level of IL-4 both were significantly higher(P<0.05)in the PBLs cultured for 0 and 48 h from children with HSP than in those from healthy controls. After 48 h of TP treatment, the percentage of CD3(+)CD25(+) cells and the level of IL-4 in the PBLs from HSP children were both decreased significantly(2玃<0.05).Conclusion There exist multiple immune regulatory disturbances in the genesis of HSP. TP may exert its therapeutic effects by inhibiting the enhanced activation and secretion of T cells in HSP patients.

关 键 词：过敏性紫癜 淋巴细胞活化 患儿 白细胞介素-4(IL-4) 外周血淋巴细胞 流式细胞仪检测 PBLS 雷公藤内酯醇 CD25抗原 分泌水平 EIISA HSP 健康儿童 异常增高 发病机制 作用机制 百分率 CD3 表达率 培养 平均 TP 

分 类 号：R725.546[医药卫生—儿科] R392.12[医药卫生—临床医学]