五羟色胺促进骨髓基质细胞生长初探  

Serotonin stimulates the growth of bone marrow stromal cells

作　　者：易岂建[1] 杨默[2] 庞雅轩[2] 李桂霞[2] 夏文杰[2] 霍泰辉[2] 宋银子[2] 

机构地区：[1]重庆医科大学儿科学院心内科,重庆400014 [2]香港中文大学儿科系

出　　处：《重庆医科大学学报》2005年第2期239-242,共4页Journal of Chongqing Medical University

基　　金：香港中文大学科学基金资助.

摘　　要：目的:探讨五羟色胺(serotonin ,5 -HT)对骨髓基质细胞(bonemarrowstromalcell,BMSC)体外生长的影响。方法:采用骨髓基质细胞集落培养技术(CFU -F)检测不同浓度5 -HT以及5 -HT分别与碱性成纤维细胞生长因子(basic-fibroblastgrowthfactor,bFGF) ,血小板源性生长因子( platelet-derivedgrowthfactor ,PDGF) ,血管内皮细胞生长因子(vascularendothelialgrowthfactor ,VEGF)对小鼠及人BMSC体外培养的生长促进作用,反转录多聚酶链反应(RT -PCR)检测人BMSC表面5 -HT2型受体mRNA表达。结果:5 -HT不同浓度( 5 0 ,10 0 ,2 0 0 ,5 0 0nM)对小鼠及人BMSC体外培养均有生长促进作用(P <0 .0 5 ) ,显示最大作用浓度为2 0 0nM。5 -HT可增强bFGF ,PDGF和VEGF对BMSC生长促进作用。人BMSC表面有5 -HT2A ,2B ,2C受体mRNA表达。结论:5 -HT具有BMSC生长促进作用,并可增强bFGF ,PDGF ,VEGF促进BMSC的生长,其作用途径可能通过BMSC表面5 -HT2型受体。Objective:To explore the effect of 5-hydroxytryptamine(5-HT) on proliferation of bone marrow stromal cells(BMSCs) in vitro.Methods:Murine BMSCs colony-forming unit assay and human bone marrow mononuclear cells count assay were used respectively to determine the effect on proliferation of BMSCs with different dose 5-HT(0,50,100,200,500nM) and 5-HT combined with basic fibroblast growth factor(bFGF),platelet-derived growth factor(PDGF),and ventricular endothelial growth factor(VEGF).Reversal transcript polymerase chain reaction(RT-PCR) was used to detect expression of mRNA of 5-HT2 receptor subtypes.Results:The results showed that 5-HT significantly stimulated murine colony-forming unit formation(CFU-F) proliferation in a dose-dependent manner(50 to 500nM) using murine bone marrow cells( n =3).Maxmum stimulation was obtained with 200nM of 5-HT( P =0.03).The effect of 5-HT on murine CFU-F proliferation was compared with that of bFGF,PDGF and VEGF at their optimal dose.The stimulating activity of 5-HT was the same as that of PDGF and VEGF,but lower than that of bFGF.A significantly enhancing effect was observed in 5-HT plus bFGF,PDGF,and VEGF,respectively.We also found that 5-HT,or 5-HT plus bFGF,PDGF,and VEGF had similar effect on human bone marrow stromal cells proliferation.There were expression of mRNA of 5-HT 2A,2B and 2C receptor subtypes on human bone marrow stromal cells.Conclusion:These data indicate that 5-HT has a significant mitogenic effect on BMSCs in vitro and enhance proliferating effect of bFGF,PDGF,and VEGF on BMSCs.This effect of 5-HT on BMSCs appears to be mediated via 5-HT2 receptor subtypes.

关 键 词：五羟色胺 骨髓基质细胞 受体 

分 类 号：R322.2[医药卫生—人体解剖和组织胚胎学]