中药肺宁对实验性肺纤维化的保护作用  

Protective effect of FeiNing on pulmonary fibrosis in a rat model

作　　者：卫张蕊[1] 田琼[1] 陈健康[1] 刘水冰[1] 高瑛[2] 周国锋[3] 

机构地区：[1]第四军医大学基础部基础医学教学实验中心,陕西西安710033 [2]第四军医大学西京医院血液科,陕西西安710033 [3]第四军医大学口腔医学院修复科,陕西西安710033

出　　处：《第四军医大学学报》2005年第7期625-627,共3页Journal of the Fourth Military Medical University

摘　　要：目的:观察中药肺宁对博莱霉素(BL)诱导的实验性小鼠肺纤维化的保护作用. 方法:以博莱霉素气管内注射建立肺纤维化模型后,第2日始分别进行龙胆花、三七总甙(PNS)、强的松和肺宁灌胃治疗,于给药第7, 56日处死动物,左肺组织行病理组织学检查,右肺组织匀浆进行超氧化物歧化酶(SOD)活力及羟脯氨酸(Hp)含量检测. 结果:与博莱霉素对照组相比:各治疗组肺泡炎和肺纤维化的程度均明显减轻; 7d时龙胆花、肺宁、强的松组肺组织匀浆SOD活力均非常显著地增高(P<0. 01),PNS组效果较差(P>0. 05 ); 56d时各治疗组Hp含量均非常显著地降低(P<0. 01). 56d时肺宁组Hp含量与正常组无显著性差异. 结论:PNS和龙胆花水煎液均可以提高SOD的活性,改善氧化/抗氧化平衡,抑制胶原沉积,保护肺组织免受纤维化损伤. 肺宁对肺的保护作用非常显著.AIM: To study the protective effect of FeiNing on Bleomycin (BL)-induced experimental pulmonary fibrosis in rats. METHODS: All the rats were randomly divided into six groups and all the rats except those in normal control group were given BL endotracheally. On d 2, each BL group was given LongDanHua, PNS, FeiNing and Hydrpnredniosone respectively. On d 7 and d 56, five rats in each group were killed and the lungs were harvested. The left lungs were used for histopathological studies (HE and MASSON staining) and the right lungs were homogenized for biochemical assays of SOD activity and Hp content. RESULTS: Compared with those in model group, the aleveolitis and pulmonary fibrosis were significantly alleviated in each treated group and the SOD activity in each treated group except that treated with PNS remarkably increased on d 7 ( P <0.01). On d 56, the Hp contents in all the treated groups were significantly lowered ( P <0.01) except those in the FeiNing treated group, which were not significantly different from those in the normal group. CONCLUSION: Both LongDanHua and PNS can enhance the activity of SOD, improve the oxidation/deoxidation balance, restrain the accumulation of collegan and protect the lungs from fibrosis. FeiNing has remarked protective effect on lungs.

关 键 词：龙胆花 三七总甙 博莱霉素 肺纤维化 肺宁 

分 类 号：R281.1[医药卫生—中药学]