反相高效液相色谱法制备喜树细胞内生物碱单体  

Preparation of Individial Alkaloids from Camptotheca AcuminataDecaisne Cells with High Performance Liquid Chromatography

作　　者：于放[1] 张冬艳[1] 关水[1] 白凤武[1] 安利佳[1] 

机构地区：[1]大连理工大学生物科学与工程系,大连116023

出　　处：《分析化学》2005年第4期501-504,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金资助项目(No. 20176058)

摘　　要：利用制备高效液相色谱法从喜树细胞中分离制备了在原植株内不存在的两种全新的生物碱单体喜树异碱A和喜树异碱B。在C18柱上,以乙腈水(体积比为35∶65)为流动相,流速为5mL/min,采用等度洗脱方式,制备了这两种化合物。经紫外光谱、红外光谱及质谱分析,对这两种化合物进行了确认。高效液相色谱分析表明,制备的喜树异碱A和喜树异碱B的纯度均在97% (质量分数)以上。在该色谱条件下,各色谱峰达到了基线分离,且分离时间短,溶剂用量少。A method using preparative high performance liquid chromatography ( HPLC) to isolate two individial alkaloids from cells of Camptotheca acuminata Decaisne was investigated. A preparative C-18 column was employed in the procedure. By isocratic elution with acetonitrile-water (35:65, V/V) as mobile phase at a flow rate of 5 mL/min, two alkaloids were obtained and subsequently were indetified by ultraviolet, infrared and mass spectroscopic methods as isocamptothecin A and isocamptothecin B. HPLC analytical results showed that the purity of each alkaloid was over 97%. Under the chromatographic conditions, every peak was completely baseline separated and consumption of solvents was low.

关 键 词：反相高效液相色谱法 生物碱 喜树 单体 细胞内 分离制备 C18柱 洗脱方式 紫外光谱 质谱分析 红外光谱 质量分数 色谱条件 基线分离 分离时间 溶剂用量 化合物 流动相 体积比 min 分析表 色谱峰 水 

分 类 号：TQ464.4[化学工程—制药化工]