两性离子表面活性剂作为共振光散射探针测定脱氧核糖核酸  

Zwitterionics Cocamidopropyl Hydroxysultaine as a New Resonance LightScattering Probe for the Determination of Deoxyribonucleic Acid

作　　者：陈展光[1] 丁卫锋[1] 韩雅莉[1] 卢峰[1] 廖栩泓[1] 陈小明[2] 

机构地区：[1]汕头大学化学系,汕头515063 [2]湘潭大学化学化工学院,湘潭411105

出　　处：《分析化学》2005年第4期519-522,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No. 30271033);汕头市科学技术委员会重点科研基金(No.S02023)资助项目

摘　　要：将两性离子表面活性剂用于DNA的共振光散射测定.在pH值为7.96～9.40较宽的范围内,DNA与椰油酰胺丙基-2-羟基-3-磺基丙基甜菜碱(HSB)在392 nm处有稳定的共振光散射增强,其强度与DNA浓度呈线性关系,建立了一种测定DNA的新方法.线性范围为0.02～4.25 mg/L;检出限达1.5 μg/L.该法简便、快速,用于合成样品中DNA的测定,重现性好,结果满意.同时探讨了有关机理.A novel method for the determination of deoxyribonucleic acid was established based on the measurement of resonance light scattering ( RLS) signals, which results from the interaction of zwitterionics cocamidopropyl hydroxysultaine ( HSB) with DNA. In the range of pH 7. 96 ∼ 9. 40, the reaction between HSB and DNA enhances the weak RLS signals of HSB, and the enhanced light scattering intensity is proportional to the concentration of DNA. The method is sensitive ( 1. 5 μ g/L), simple with wide linear range. ( 0. 02 ∼ 4. 25 mg/L). Synthetic samples have been determined with. satisfactory results.

关 键 词：两性离子表面活性剂 共振光散射探针 脱氧核糖核酸 测定 共振光散射增强 丙基甜菜碱 DNA浓度 酰胺丙基 线性关系 线性范围 合成样品 pH值 检出限 重现性 磺基 羟基 

分 类 号：Q523[生物学—生物化学]