DNA数目可变串联重复序列用于结核分枝杆菌分型研究  

Study on molecular typing of Mycobacterium tuberculosis with variable number of tandem repeats

作　　者：阚晓宏 万康林[2] 金玉莲[1] 刘敬华[2] 杨建安[1] 刘志广[2] 丁虹[1] 赵秀琴[2] 王庆[1] 唐婧[1] 

机构地区：[1]安徽省结核病防治研究所,合肥230022 [2]中国疾病预防控制中心传染病预防控制所,北京102206

出　　处：《中国防痨杂志》2005年第2期77-81,共5页Chinese Journal of Antituberculosis

基　　金：安徽省卫生厅科研基金课题 (编号 :2 0 0 2A0 3 0 );国家"十五"重点攻关项目 ;中国重要医学生物资源的保藏与共享--我国重要细菌菌种的收集;保藏与共享 (编号 :2 0 0 1DEA10 0 0 7)的资助。

摘　　要：目的　采用数目可变串联重复序列 (VNTR)分型方法对安徽省的 5 2株结核分枝杆菌进行分子分型研究 ,探讨该方法用于结核分枝杆菌分型的作用。方法　设计引物 ,采用PCR和琼脂糖凝胶电泳技术对结核分枝杆菌 13个VNTR位点进行检测 ,并通过BioNumerics 3 0软件进行DNA指纹图谱多态性分析。结果　 5 2株结核分枝杆菌可分 4个类别 ,其中 88. 5 %菌株属于一个型 ,其他 3个型所占比例很小 ,分别为 5 . 8%、3 .8%和 1 .9%。结论　来自安徽的结核分枝杆菌存在主要的流行型 ,VNTR分型技术简便、快速 ,是较好的结核分枝杆菌分型方法。Objective To study on molecular typing of 52 Mycobacterium tuberculosis (MTB) isolates from the patients with tuberculosis in Anhui province by variable number of tandem repeats (VNTR) method, and to evaluate the value of the genotyping method in genotyping of MTB. Methods Thirteen tandem repeat loci in the total genome of MTB were analyzed by PCR and agarose gel electrophoresis method. The character of the polymorphism of DNA fingerprinting of 52 MTB strains was analyzed with BioNumerics 3 0 software. Results The 52 MTB strains were finally classified into 4 genotypes. Most (88 5%) of them belonged to one genotype, the other three genotypes scattered, accounted for 5 8%, 3 8% and 1 9% strains, respectively. Conclusion The results showed that there perhaps exists epidemic strain clusters in MTB strains from Anhui province. This fast and simple technique, VNTR method, is better molecular typing method for MTB Genotyping.

关 键 词：结核分枝杆菌 数目可变串联重复序列 基因分型 琼脂糖凝胶电泳技术 

分 类 号：R346[医药卫生—基础医学]