微分析系统对苯二酚抑制化学发光的研究  

Study of inhibited chemiluminescence reaction by hydroquinone on microchip

作　　者：徐溢[1] 兰宇卫[1] 张晓凤[1] 吕昊[1] 

机构地区：[1]重庆大学化学化工学院

出　　处：《分析试验室》2005年第4期33-36,共4页Chinese Journal of Analysis Laboratory

基　　金：国家教委留学回国人员基金资助 (1999 36 3);国家自然科学基金资助 (2 0 0 0 70 0 5 );教育部光电技术及系统教育部重点实验室访问学者基金资助

摘　　要：在自行设计和制作的有机玻璃 (PMMA)微流控芯片上 ,以流动注射方式进样 ,利用对苯二酚对Luminol H2 O2 Co2 +体系化学发光 (CL)的强抑制作用 ,建立了间接测定对苯二酚含量的微型全化学分析系统 ( μ TAS)。对影响化学发光反应的各因素进行优化 ,对芯片结构的设计和反应过程进行探讨 ,设计并制作出适合该反应体系的芯片。在合成水样对苯二酚的测定中 ,化学发光强度的变化与对苯二酚的浓度在 1 .0× 1 0 - 7～ 2 .0× 1 0 - 8mol/L范围内成良好线性关系 ,检出限达 2 .0× 1 0 - 9mol/L。The miniaturized total analytical system (μTAS) which indirectly detected the concentration of hydroquinone was established by means of flow injection analysis (FIA) technique and the inhibited reaction of hydroquinone to Luminol-H_2O_2-Co^(2+) chemiluminescence on designed PMMA microchip. Different factors affecting the inhibited CL reaction, especially concentration of hydroquinone, were optimized. In order to design a microchip suitable to this reaction system, the design of chip configuration and the process of these reactions were studied. The chemiluminescence intensity had a linear relation ship with the concentration of hydroquinone in water samples, and the detection limit of this method was 2.0×10^(-9) mol/L and the linear range was between 2.0×10^(-7) and 2.0×10^(-8) mol/L.

关 键 词：微型全化学分析系统(μTAS) 流动注射分析 抑制化学发光 对苯二酚 

分 类 号：O657.32[理学—分析化学]