微渗析活体取样与液相色谱安培检测法联用测定鼠脑中黄嘌呤和次黄嘌呤  

In vivo determination of xanthine and hypoxanthine in rat striatum by microdialysis sampling coupled with high performance liquid chromatography amperometric detection

作　　者：林丽[1] 李永[1] 陈大茴[1] 仇佩虹[1] 曹旭妮[2] 金利通[2] 

机构地区：[1]温州医学院化学教研室,温州325035 [2]华东师范大学化学系,上海200062

出　　处：《分析试验室》2005年第4期43-45,共3页Chinese Journal of Analysis Laboratory

摘　　要：采用微渗析活体取样技术和高效液相色谱安培检测法 ,测定了鼠脑纹状体中的黄嘌呤和次黄嘌呤。安培检测以玻碳电极为工作电极 ,检测电位为 0 .9V。在 5 .0× 1 0 - 7～ 1 .0× 1 0 - 4 mol/L浓度范围内 ,黄嘌呤和次黄嘌呤的浓度分别与峰电流呈良好的线性关系 ,检出限分别为 8 0× 1 0 - 8mol/L和 3 0× 1 0 - 7mol/L。Microdialysis sampling coupled with high performance liquid chromatography and amperometric detector was developed and applied to determine Xanthine and Hypoxanthine in rat striatum. A glass carbon electrode was used as the working electrode for amperometric detector. The working potential was 0.9 V. The results indicated that the peak currents of Xanthine and Hypoxanthine were linear to their concentrations ranging from 5.0×10^(-7) to 1.0×10^(-4) mol/L with the calculated detection limits of 8.0×10^(-8) mol/L and 3.0×10^(-7) mol/L, respectively.

关 键 词：微渗析活体取样 高效液相色谱安培检测 黄嘌呤 次黄嘌呤 

分 类 号：O657.7[理学—分析化学]