气道黏液高分泌诱导因素的研究  

A comparative investigation of induction factors of airway mucus hypersecretion

作　　者：聂晓红[1] 周向东[1] 

机构地区：[1]重庆医科大学附属第二医院呼吸科,400010

出　　处：《中国药物与临床》2005年第1期26-28,共3页Chinese Remedies & Clinics

基　　金：重庆市医学科技计划资助项目(2003-B-32)

摘　　要：目的比较不同因素对气道黏液高分泌的诱导作用,为动物模型制造和临床防治提供指导。方法Wistar大鼠30只随机分成3组:正常组雾化吸入生理盐水;对照组雾化吸入脂多糖(LPS);试验组雾化吸入中性粒细胞弹性蛋白酶(NE)。采用酶联免疫吸附试验(ELISA)、免疫组织化学和斑点印迹法分别测定支气管肺泡灌洗液中黏蛋白(MUC)的含量、气道上皮MUC5AC以及MUC5AC mRNA表达。结果试验组和对照组比正常组的MUC,MUC 5AC以及MUC5AC mRNA水平均显著增加(P<0.01);试验组MUC,MUC5AC以及MUC5AC mRNA表达较对照组明显增加(P<0.05)。结论LPS和NE均为黏液高分泌的有效诱导因素,但NE的黏液促分泌效应较LPS更显著,为强有力的诱导剂,是理想的黏液高分泌造模因子,也是临床防治的重要靶目标。Objective To compare the inducing effects of different secretagogues on hypersecretion of respiratory mucosa. Methods Thirty Wistar rats were randomly divided into 3 groups,ie, normal group, control group and trial group;they were put into lucite bins and inhaled saline(0.9%NS), lipopolysaccharide(LPS)and neutrophil elastase (NE) respectively. The levels of mucins (MUC) in bronchoalveolar lavage fluid (BALF),MUC5AC in airway epithelia and MUC5AC mRNA in lung tissues were determined using ELISA, immunohistochemistry and dot blot assay respectively. Results The content of MUC, the positive stain area of MUC5AC and the express of MUC5AC mRNA in the trial group and control group were significantly increased in comparison with the normal group(P <0.01), while the three above-mentioned items in trial group were apparently increased in comparison with the control group(P <0.05). Conclusion LPS and NE are mucus secretagogues. NE has stronger inducing effect on mucus secretion than LPS, it is a potent secretagogue and an ideal agent for setting up respiratory mucous hypersecretion model.NE is also a important target of prevention and cure in the clinic.

关 键 词：黏液高分泌 诱导因素 酶联免疫吸附试验(ELISA) MUC5AC 中性粒细胞弹性蛋白酶 WISTAR大鼠 脂多糖(LPS) 支气管肺泡灌洗液 mRNA表达 雾化吸入 免疫组织化学 临床防治 斑点印迹法 对照组 诱导作用 动物模型 生理盐水 气道上皮 

分 类 号：R56[医药卫生—呼吸系统]