睡眠呼吸暂停综合征致动脉内皮损伤的电镜研究  

Electron microscope studies on artery endotheliocyte of experimental sleep apnea syndrome in rats

作　　者：王璋[1] 司良毅[1] 

机构地区：[1]第三军医大学西南医院老年病科,重庆400038

出　　处：《重庆医学》2005年第4期556-557,559,共3页Chongqing medicine

基　　金：中央保健局保健专项资金重点资助重庆市干部保健委员会(渝A040)

摘　　要：目的　研究睡眠呼吸暂停综合征加高脂饮食大鼠动物模型动脉粥样硬化(AS)的病理改变。方法　选取Wistar雄性青年大鼠〔3月龄,体重(250±30)g〕与老年大鼠〔20月龄,体重(450±50)g〕各24 只。各组随机分为对照组、高脂组及叠加组,每组8只。实验时间12周,实验终点检测空腹血清的血脂水平;观察胸主动脉内皮病变的电镜特征。结果　高脂饮食组与叠加组均出现高脂血症。高脂饮食组出现轻度内皮损伤,叠加组内皮病理损伤较高脂组严重,形成较典型的AS早期病变,包括内皮细胞脱失,平滑肌细胞增殖、迁移,吞噬脂质空泡等。结论　睡眠呼吸暂停综合征可加速高脂血症大鼠AS早期病变的进程。Objective To investigate the histopathological and ultrastructural features of the experimental atherosclerosis model using cholesterol fed rats of sleep apnea syndrome.Method Twenty four Male young Wistar rats(3 months old, 250±30g)and twenty four Senium Wistar rats (20 months old, 450±50g) were randomly divided into 3 groups (n=8). The first group was fed by normal diet as empty control, the second group were fed by high cholesterol diet as cholesterol fed control group, the third group were fed by high cholesterol diet and simulated as sleep apnea syndrome by chronic intermittent hypoxia exposed.At the end of experiment, blood serum of rats was detected by automatic biochemistry analyzer for cholesterol. The thoracic aorta were examined by electron microscopy.Results (1)Both group 2 and 3 rats showed hyperlipemia. Statistical analysis showed an obvious difference contrast with control group( P<0.01). The level of TC and TG between group 2 and 3 rats did not show an obvious difference(P>0.05).(2)Light lesion of artery endodermis was observed in the cholesterol fed groups. The typical lesion of atherosclerosis earlier period was observed only in the groups 3. The changes included endothelial exfoliation significant,the proliferation and migration of smooth muscle cells,the accumulation of lipid and cholesterol in the associated cells and.Conclusion This study shows that the typical thoracic aorta atherosclerosis lesion can be induced by chronic intermittent hypoxia exposed in the cholesterol fed rats.

关 键 词：睡眠呼吸暂停综合征 动脉粥样硬化 电镜 

分 类 号：R56[医药卫生—呼吸系统]