骨髓基质细胞移植对大鼠损伤脊髓GDNFmRNA表达的影响  

Influence of BMSCs transplantation on expression of GDNF mRNA in spinal cord injury of rats

作　　者：李雷[1] 吕刚[2] 王岩峰[2] 高红[3] 许欣 白伦浩[1] 王欢[1] 

机构地区：[1]中国医科大学附属第二医院骨科,辽宁沈阳110004 [2]中国医科大学附属第一医院骨科,辽宁沈阳110004 [3]中国医科大学附属第二医院小儿先天畸形实验室,辽宁沈阳110004 [4]中国医科大学基础医学院发育生物学研究室

出　　处：《中国医科大学学报》2005年第2期102-104,共3页Journal of China Medical University

基　　金：辽宁省教育厅高等学校研究项目(2004F072)

摘　　要：目的:研究骨髓基质细胞(BMSCs)移植对大鼠损伤脊髓胶质细胞源性神经营养因子(GDNF)表达的影响。方法:将大鼠36只随机分为移植组和对照组,每组3个时相点,每个时相点每组6只动物。移植或手术后24, 72h和7d, 用RT PCR方法对GDNF的表达进行半定量分析。结果:移植组GDNFmRNA24, 72h和7d时都明显高于单纯损伤组(P<0 .05)。结论:BMSCs移植可使损伤脊髓表达GDNF增强。Objective: To explore the influence of bone marrow stromal cells(BMSCs)transplantation on expression of gliocyte derived neurotrophic factor(GDNF) mRNA in spinal cord injury of rats.Methods:Thirty-six rats were divided into transplantation group and control group.There were 3 time points in each group.The expression of GDNF mRNA were evaluated by using RT-PCR after transplantation or operation. Results:The expression levels of GDNF mRNA in transplantation group were significantly higher than those in control group at the 24-hour,72-hour,and 7-day time points.Conclusion:The transplantation of BMSCs can elevate the expression levels of GDNF mRNA in spinal cord injury of rats.

关 键 词：脊髓损伤 骨髓基质细胞 移植 神经营养因子 大鼠 

分 类 号：R622[医药卫生—整形外科]