定量聚合酶链式反应技术检测骨关节炎软骨组织中纤维连接蛋白mRNA的表达水平  

Determination of fibronectin mRNA expression in tissue of osteoarthritis with quantitative polymerase chain reaction technique

作　　者：路宝民[1] 范广宇[2] 马汝海[3] 

机构地区：[1]辽宁省人民医院骨科 [2]中国医科大学附属第一医院骨科,辽宁沈阳110001 [3]中国医科大学基础医学院化学教研室

出　　处：《中国医科大学学报》2005年第2期123-124,共2页Journal of China Medical University

摘　　要：目的: 探讨纤维连接蛋白(FN)mRNA表达水平的变化与骨关节炎的关系。方法:采用石膏管型固定右膝关节,人工制作骨关节炎动物模型作为实验组,另设对照组,采用定量聚合酶链式反应方法检测FNmRNA表达水平的变化。结果:Wistar鼠正常膝关节的软骨组织与人工制造膝关节骨关节炎的软骨组织中均存在FNmRNA表达,但其表达水平在骨关节炎的软骨组织中显著低于正常膝关节的软骨组织(P<0.01)。结论:软骨组织中FAmRNA表达水平降低可能是骨关节炎发生和难以愈合的原因之一。Objective: To study the relationship between the expression of fibronectin mRNA and osteoarthritis. Methods: Wistar rats in experimental group received joint immobilization .The right knee joints were fixed to make model of osteoarthritis. The expression of fibronectin mRNA was determined by using quantitive polymerase chain reaction. The result was compared with that of control group. Rusults: The expression of fibronectin mRNA was found in both 2 groups.However,the expression in cartilage tissue of experimental group was significantly lower than that of control group (P< 0.01). Conclusion: The expression of fibronectin mRNA decreased in cartilage tissue of osteoarthritis.

关 键 词：骨关节炎 纤维连接蛋白 定量聚合酶链式反应 

分 类 号：R684.3[医药卫生—骨科学]