小鼠局灶性脑缺血梗死灶的定量分析  

Quantitative analysis of focal ischemic cerebral infarction in mice

作　　者：俞月萍[1] 徐秋琴[2] 张琦[1] 郑鸣之[1] 胡珏[1] 魏尔清[3] 

机构地区：[1]浙江医学高等专科学校药理学教研室 [2]杭州师范学院医学院药理学教研室 [3]浙江大学医学院药理学教研室

出　　处：《中国临床药理学与治疗学》2005年第3期352-356,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：浙江省科技计划项目 (№ 2 0 0 2C3 3 0 5 6) ;浙江省医药卫生科研基金项目 (№ 2 0 0 2B0 14 )

摘　　要：目的:用小鼠持续性局灶性脑缺血模型,证明新建的透光法测定局灶性脑缺血梗死灶的实用性。方法:采用大脑中动脉阻塞法(MCAO)造成小鼠持续性局灶性脑缺血,于缺血后2 4h进行Bederson’s症状评分和爬板、悬挂试验,并以计算机图像分析技术测定和分析脑缺血梗死体积、脑半球面积、皮层及皮层下神经元密度;在大脑中动脉线栓手术前3d和术前1h分别腹腔注射Pranlukast 0 .1mg·kg-1或尼莫地平0 .4mg·kg-1,观察药物的神经保护作用。结果:透光测定的梗死体积与TTC染色测定的梗死体积、神经元密度密切相关,与神经症状综合评分具有等级相关。Pranlukast和尼莫地平能减少脑梗死体积和脑半球的缺血侧 非缺血侧比值,减轻神经症状和神经元死亡。结论:透光法结合神经症状综合评分法可用于小鼠局灶性脑缺血的定量分析和药物的神经保护作用评价。AIM:To confirm the action of the light transmission method in evaluating focal ischemic cerebral infarction on persistent focal cerebral ischemia in mice. METHODS:Persistent focal cerebral ischemia was induced by middle cerebral artery occlusion (MCAO). Bederson's neurological scores, climbing board and hanging test were performed 24 h after ischemia, and infarct volume, brain hemisphere area, neuron density of cortex and subcortex were measured with computer-assisted imaging. Pranlukast ( 0.1 mg·kg -1) or nimodipine ( 0.4 mg·kg -1) were injected ip once daily for 3 days and to 1 h before MCAO assess the neuroprotective effect. RESULTS:The infarct volumes measured by light transmission closely correlated with that measured by TTC staining and neuron densities. The infarct volumes measured by light transmission well correlated with the neurological scores measured by integrated graded approach, too. Both pranlukast and nimodipine significantly attenuated infarct volumes and the ratio of ischemic/non-ischemic hemispheres, and reduced neurological deficits and neuron death. CONCLUSION:Light transmission and integrated graded approach can be used not only for qualitative analysis of focal cerebral ischemia, but also for evaluating the neuroprotective effect of drugs.

关 键 词：大脑中动脉阻塞 局灶性脑缺血 脑梗死 脑水肿 透光法 

分 类 号：R965[医药卫生—药理学]