不同有色膜对丰香草莓再生的影响及机理研究  

Regeneration Mechanism of Fragaria×ananassa Duch. cv. Toyonoka Under Different Color Plastic Films

作　　者：秦永华 [1] ZHANG Shang-long  秦巧平 [1] 吴延军 [2] 徐凯 [1] 

机构地区：[1]浙江大学园艺系/农业部园艺植物生长发育与生物技术重点开放实验室,杭州,310029 [2]浙江大学园艺系/农业部园艺植物生长发育与生物技术重点开放实验室,杭州,310029,安徽农业大学园艺系,合肥,230036

出　　处：《中国农业科学》2005年第4期777-783,共7页Scientia Agricultura Sinica

基　　金：中国科学院资助项目

摘　　要：以丰香草莓叶片为材料,MS附加1.5 mg·L-1TDZ和0.4 mg·L-1IBA为基本培养基,研究了不同有色膜对草莓再生的影响,并对其机理进行了探讨.结果表明,绿膜和红膜对不定芽再生有明显的促进作用,其再生率达95%以上,平均每个外植体再生芽数25个以上;而蓝膜和黄膜则不利于芽的分化.分析发现,不同有色膜光波差异主要集中在300～700 nm,红、绿膜在该波段光强较弱,而黄、蓝膜和荧光下较强.红膜和绿膜有较高的叶绿素含量,较低的Chl a/b比值.抗氧化酶的活性以红膜最高,绿膜其次,黄膜最低;而丙二醛(MDA)的含量则相反.内源激素与草莓离体器官的发生之间关系密切,培养15 d后,红、绿膜和荧光下赤霉素(GA3)的含量较高;45 d后,GA3和玉米素(ZT)的含量以红、绿膜处理最高,而吲哚乙酸(IAA)的含量以黄膜和蓝膜下的较高.The effect of different color plastic films on the shoot regeneration and its mechanism from the leaf discs of Fragaria×ananassa Duch. cv. Toyonoka were studied in an experiment using an optimized MS medium (1.5 mg·L–1 TDZ and 0.4 mg·L–1 IBA). Color films had significant effects on shoot regeneration of strawberry. The highest percentage of shoot regeneration (> 95%) and maximum number of shoots per explant (>25) were observed under red and green films. Different spectrum ranges of the color films were 300 to 700 nm. Light intensity under red and green films was weaker than fluorescent light and yellow, blue films. Red and green films induced a higher level of chlorophyll and lower chlorophyll a/b in tissue culture of strawberry. Antioxidant enzyme (SOD, CAT and POD) activities were the highest under red films, followed by green and the lowest was under yellow ones. Antioxidant activities had positive correlation with the shoot regeneration while malondialdehyde (MDA) was negative. After 15 days in culture, GA3 content was higher under fluorescent light, red and green films, respectively. Zeatin (ZT) and gibberellic acid (GA3) contents were the highest under red and green films after 45 days, however, indole-3-acetic acid (IAA) accumulation in the tissues was higher under yellow and blue films.

关 键 词：有色膜 丰香草莓 植株再生 发生机理 丙二醛 抗氧化酶 赤霉素 吲哚乙酸 玉米素 

分 类 号：S668.4[农业科学—果树学]