乙型肝炎病毒前C/C区及其调控基因变异的研究  

Gene variations in the basic core promotor and the pre C/C region of hepatitis B virus

作　　者：梁蔚芳[1] 何海棠[1] 刘志华[1] 骆抗先[1] 

机构地区：[1]南方医科大学南方医院感染内科,广州510515

出　　处：《解放军医学杂志》2005年第4期331-334,共4页Medical Journal of Chinese People's Liberation Army

摘　　要：目的研究慢性乙型肝炎病人中HBVpreC/C基因及其调控序列的变异和特点。方法对42例慢性乙型肝炎病人中扩增的HBVDNA各3个克隆进行序列分析。结果42例病人,HBVC基因调控序列发生T1762A1764双突变者20例,其中HBeAg阳性者7例,HBeAg阴性者13例(P<005);22例发生T1673G1799双突变。前C变异中,18例发生A1896变异,其中HBeAg阳性者6例,HBeAg阴性者12例(P<0.05)。C区变异中,AA5、AA38、AA60、AA87、AA97、AA130、AA135都是变异的热点。前C/C区还存在有插入、缺失等不同变异。结论慢性乙型肝炎病人的HBVC基因及其调控序列变异具有多样性及复杂性,与体内的免疫清除与病毒逃避免疫攻击相关,从而容易导致疾病的慢性化。Objective To study the mutants of pre C/C gene and the base core premotor (BCP) in chronic hepatitis B patients carrying or not carrying hepatitis B e antigen (HBeAg). Method The HBV DNA of BCP and PreC/C ware amplified by PCR from serum samples of patients with chronic hepatitis B, and the products were cloned into T vector and sequenced. Results Of BCP variants, the double mutations of T1762/ A1764 were found in 20 of 42 patients with chronic hepatitis, and the prevalence was significantly lower in HBeAg positive patients (7/20) than in HBeAg negative patients (13/22) (P<0.05). Double variants of T1673G1799 were found in 22 of 42 patients. Precore variant, A1896, was detected in 18 individuals, 6 of them were positive for HBeAg and 12 were negative (P<0.05). Core variations, AA5, AA38, AA60, AA87, AA97 and AA135 were the hot spots of mutation. There were other mutants in preC/C such as inserts, deletions, etc. Conclusion The mutations in the BCP and the preC/C region were multiform and complex, implying that these mutations might be related to the immune clearance and ability of the virus to elude attacks, resulting in inveterate course of the disease.

关 键 词：肝炎病毒 乙型 基因 变异 

分 类 号：R512.62[医药卫生—内科学]