家蚕催青前期胚胎蛋白质双向电泳图谱分析  

Analysis of protein patterns from embryo of silkworm Bombyx mori at earlier stage by two dimensional polyacrylamide gel electrophoresis

作　　者：颜新培[1] 钟伯雄[1] 徐孟奎[1] 梁建设[2] 沈飞英[1] 

机构地区：[1]浙江大学动物科学学院蚕蜂分子生物学实验室,杭州310029 [2]浙江大学生物化学有限公司,杭州310029

出　　处：《昆虫学报》2005年第2期295-300,共6页Acta Entomologica Sinica

基　　金：国家自然科学基金项目 (30271004)

摘　　要：为了探讨家蚕 Bombyx mori胚胎蛋白质整体变化, 以多化性品种P50为材料, 采用蛋白质双向电泳技术及图像分析技术分析了催青前期胚胎 (戊3 以前) 各个时期蛋白质图谱及其变化情况。研究发现: 从临界Ⅱ期 (丙2 ) 胚胎到缩短期(戊2) 胚胎蛋白质双向电泳图谱基本稳定, 存在于临界Ⅱ期胚胎的蛋白斑点在催青前期的4个胚胎中消失的个数较少, 仅占22 80%, 而在催青的最后2个胚胎中消失的蛋白质斑点却占 48 18%; 在神经沟出现 (丁1)、腹肢突起 (丁2)、上唇突起 (戊1) 和缩短期 (戊2) 胚胎的双向电泳图谱中能够检测到 100 个特异蛋白质斑点, 这些特异蛋白质斑点大多在随后邻近的胚胎发育中消失, 暗示了这些特异蛋白可能与相应胚胎的形体特征发育有关。The proteins during earlier stages of embryo (before the head thorax differentiation embryo) and their changes were analyzed using silkworm Bombyx mori variety P50 as experimental material by two dimensional polyacrylamide gel electrophoresis and image analysis system to analyze the change of total proteins from silkworm embryo. It was discovered that two dimensional polyacrylamide gel electrophoresis patterns of protein from the critical development Ⅱ stage embryo to the shortening stage embryo stayed basically stable, and only a small number of the protein spots existed in the critical development Ⅱ stage embryo disappeared at four earlier embryo stages of incubation, which only accounted for 22.80%, but the protein spots which disappeared at 2 last embryos of incubation accounted for 48.18%; 100 different protein spots from the neural groove appearance, abdominal outgrowth appearance, labrum appearance and shortening stage embryo can be detected and most of these protein spots disappeared in the embryos closely followed. The results suggested these different proteins might relate to the characteristics of corresponding embryo body development.

关 键 词：家蚕 蛋白质 双向电泳 图谱分析 胚胎发育 

分 类 号：S881.2[农业科学—特种经济动物饲养]