鱼藤酮致PC12细胞线粒体功能及超微结构的影响  

Mechanism of mitochondrial dysfunction and ultrastructural changes induced by rotenone in PC12 cells

作　　者：赵黔鲁[1] 柴锡庆[1] 

机构地区：[1]河北医科大学研究生学院,石家庄050017

出　　处：《第三军医大学学报》2005年第7期632-635,共4页Journal of Third Military Medical University

摘　　要：目的　检测不同浓度鱼藤酮对体外培养大鼠肾上腺嗜铬细胞瘤(pheochromaffinoma ,PC12 )细胞活性氧(reac tiveoxygenspecies ,ROS)、线粒体跨膜电位(用ΔΨm表示)及超微结构的作用,以探讨鱼藤酮损害线粒体的可能机制。方法　应用流式细胞仪检测鱼藤酮对PC12细胞ROS的影响,激光扫描共聚焦显微镜(laserscanningconfocalmicroscope ,LSCM)检测线粒体跨膜电位及透射电镜观察超微结构的变化。结果　0 1、1 0、2 0和3 0 μmol/L浓度的鱼藤酮可诱导细胞产生ROS分别为1 5 5±0 17、2 16±0 10、1 77±0 2 0和1 40±0 12 ,损坏ΔΨm分别为93 86±10 12、119 43±7 0 9、10 2 71±9 3 6和83 14±10 70 ,与其对照组比较具有显著性差异(P <0 0 1) ,实验组中以1 0 μmol/L鱼藤酮作用最显著(P <0 0 1)。当鱼藤酮浓度大于1 0 μmol/L时,其作用呈现剂量依赖性递减。电镜下多数细胞线粒体形态异常,表现为:局部水肿、部分线粒体嵴排列紊乱、线粒体髓样变及空泡样变性等。结论　鱼藤酮诱导ROS生成、损伤线粒体ΔΨm及改变线粒体超微结构,可导致线粒体功能障碍。Objective To observe the effects of different doses of rotenone on reactive oxygen species, mitochondrial transemembrane potential as well as mitochondrial ultrastructure in order to investigate the possible mechanism of mitochondrial dysfunction in cultured pheochromaffinoma cells. Methods ROS production induced by rotenone in cells were measured using flow cytometry. Mitochondrial transmembrane potential was detected by laser scanning confocal microscope and mitochondrial ultrastructures were observed under transmission electron microscope. Results Treatment with 0.1, 1.0, 2.0 and 3.0 μmol/L concentrations of rotenone could increase the generation of ROS in PC12 cells as 1.55±0.17, 2.16±0.10, 1.77±0.20 and 1.40±0.12, and disrupt the mitochondrial transmembrane potential about 93.86±10.12, 119.43±7.09, 102.71±9.36 and 83.14±10.70. There were significant differences as compared with the control group (P<0.01) respectively. Among experimental groups, 1.0 μmol/L rotenone had the most significant effects. As the concentrations of rotenone got higher, the effects decreased in a dose-dependent manner. Most of mitochondrial ultrastructures obviously changed, including local edema, disarrangement of cristae, mitochondrial pupiform and vacuolate degeneration. Conclusion Rotenone may induce ROS production, harm mitochondrial membrane potential and cause changes of mitochondrial ultrastructure, resulting in mitochondrial dysfunction.

关 键 词：鱼藤酮 帕金森病 活性氧类 线粒体跨膜电位 显微镜检查 电子 

分 类 号：R329.27[医药卫生—人体解剖和组织胚胎学] R979.8[医药卫生—基础医学]