人脐血基质细胞自发分泌多种造血生长因子的实验研究  

Multiple types of hematopoietic growth factors secreted spontaneously by human umbilical cord blood stromal cells in vitro

作　　者：刘耀[1] 陈幸华[1] 张曦[1] 彭贤贵[1] 

机构地区：[1]第三军医大学新桥医院血液内科,重庆400038

出　　处：《第三军医大学学报》2005年第7期643-645,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目 ( 30 0 70 32 7) ;第三军医大学基金资助项目 ( 2 0 0 3B0 96 )~~

摘　　要：目的　探讨体外培养的人脐血基质细胞分泌血小板生成素(thrombopoietin ,TPO)、粒 巨噬细胞集落刺激因子(granulocyte macrophagecolonystimulatingfactor,GM CSF)、干细胞生长因子(stemcellfactor ,SCF)的能力。方法　留取不同培养时相点的培养液上清,ELISA法检测上清中TPO、GM CSF、SCF的浓度,并与骨髓基质细胞的培养上清液对照比较其特点。结果　人脐血基质细胞和骨髓基质细胞均可分泌TPO、GM CSF和SCF等造血生长因子,二者造血生长因子的分泌随着培养时间的延长而逐渐下降,其中人脐血基质细胞分泌TPO和SCF的高峰期出现在第7天,而GM CSF的分泌则无高峰期的出现;通过与骨髓基质细胞分泌三种造血生长因子量进行对比发现,人脐血基质细胞SCF和GM CSF的分泌量低于骨髓基质细胞,而TPO的分泌高于同期培养的骨髓基质细胞。结论　人脐血基质细胞与骨髓基质细胞一样具有分泌造血生长因子,支持调控造血的作用。Objective To investigate the potential of stromal cells from human umbilical cord blood cultured in vitro to secrete hematopoietic growth factors. Methods The expressions of hematopoietic growth factors of stromal cells from human umbilical cord blood and bone marrow were detected by ELISA kits at different time points. Results Stromal cells from human umbilical cord blood could secrete thrombopoietin (TPO), granulocyte-macrophage colony stimulating factor (GM-CSF) and stem cell factor (SCF). The peak levels of TPO and SCF were found at 7 d after culture in vitro, but then the levels of these factors decreased gradually. Stromal cells from human umbilical cord blood secreted more SCF and GM-SCF, but less TPO than human bone marrow stromal cells. Conclusion Our results demonstrate that stromal cells from human umbilical cord blood could sustain hematopoiesis by secreting multiple types of hematopoietic growth factors such as TPO, GM-CSF, and SCF.

关 键 词：人脐血基质细胞 造血生长因子 骨髓基质细胞 

分 类 号：R329.21[医药卫生—人体解剖和组织胚胎学] R331.1[医药卫生—基础医学]