清开灵有效组分透过体外模拟血脑屏障的研究  

Permeation of effective components of Qingkailing through an in vitro blood-brain barrier

作　　者：高永红 王永炎[2] 肖盛元 叶丽亚[4] 娄晋宁[4] 罗国安[3] 王硕仁 

机构地区：[1]北京中医药大学中医内科学教育部重点实验室,北京100700 [2]中国中医研究院,北京100700 [3]清华大学药物研究所,北京100084 [4]中日友好医院临床研究所,北京100029

出　　处：《北京中医药大学学报》2005年第2期26-28,共3页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家重点基础研究发展规划项目(No.G1999055404)

摘　　要：目的检测清开灵有效组分在体外模拟血脑屏障中的透过率。方法应用脑微血管内皮细胞单层培养模型体外模拟血脑屏障,用高效液相色谱-质谱(LC/MS)分析方法,检测清开灵有效组分栀子苷、黄芩苷、牛胆酸、猪去氧胆酸、去氧胆酸在24h内的透过率。结果透过率从高到低依次为栀子苷、牛胆酸、猪去氧胆酸和去氧胆酸。在滤液中未检测到黄芩苷。结论清开灵有效组分在体外模拟血脑屏障中有较高的透过率,其透过机制和药理作用值得进一步研究。Objective To determine the permeation rate of effective components of Qingkailing (ECQ) through an in vitro blood-brain barrier (BBB) model. Method To establish an analog in vitro BBB by using mouse model of microvascular endothelial cells monostratal culture. The permeation rate of ECQ (including geniposide, baicalin, cholic acid, hyodeoxycholic acid and dexocholic acid) in 24 hours is detected by means of LC/MS analysis. Result The order of permeation rate is geniposide, cholic acid, hyodeoxycholic acid and dexocholic acid. Baicalin is not detected in the colature. Conclusion The effective components of Qingkailing have a higher permeation rate in an analog in vitro BBB. The mechanism and pharmacological effect are worth studying further.

关 键 词：清开灵 有效组分 血脑屏障 透过率 

分 类 号：S285.5[农业科学—农业水土工程]