脑疏宁对颅脑创伤后大鼠血脑屏障通透性及MMP-9表达的影响  

Effects of Naoshuning on permeability of blood-brain barrier and MMP-9 expression in TBI rats

作　　者：崔向宁[1] 尹岭[2] 王玉来[1] 

机构地区：[1]北京中医药大学东方医院,北京100087 [2]解放军总医院神经信息中心,北京100853

出　　处：《北京中医药大学学报》2005年第2期60-62,i003,共4页Journal of Beijing University of Traditional Chinese Medicine

摘　　要：目的观察中药脑疏宁对创伤性脑损伤(TBI)大鼠血脑屏障(BBB)通透性及脑组织基质金属蛋白酶9(MMP9)蛋白表达的影响,探讨中药保护血脑屏障的作用机理。方法按Feeney自由落体撞击法造成TBI模型,采用免疫组织化学技术观察大鼠脑组织MMP9表达。结果模型组创伤后24hBBB破坏,脑组织含水量增加,病灶区MMP9蛋白表达增高,脑疏宁组BBB通透性、脑组织含水量及MMP9表达较模型组降低(P<0.01)。结论颅脑损伤后MMP9表达增高,促使BBB破坏,导致血管源性脑水肿。中药脑疏宁可以保护BBB,降低脑组织含水量,其作用可能与抑制MMP9在损伤脑组织中的表达有关。Objective To investigate the effect of Naoshuning on permeability of blood-brain barrier (BBB) and protein expression of matrix metalloproteinase-9 (MMP-9) in rats with traumatic brain injury (TBI), and to discuss the protective mechanism of Chinese medicine on BBB. Method The model of TBI was established by bumpiness of free falling body. Immunohistochemistry was used to observe the brain protein expression of MMP-9.Result BBB was damaged and the hydrous content of brain and the protein expression of MMP-9 were significantly increased at 24h after TBI in contusion tissue in the model group. The permeability of BBB, hydrous content of brain and the MMP-9 expression were significantly decreased in the Naoshuning group (P<0.01, P<0.01, P<0.01).Conclusion A higher MMP-9 expression can damage BBB and induce the cerebral edema after TBI. Naoshuning can protect BBB and decrease the hydrous content of brain. The mechanism may be related to its inhibiting effect on the expression of MMP-9 of TBI.

关 键 词：脑疏宁 创伤性脑损伤 血脑屏障 脑组织基质金属蛋白酶-9 大鼠 

分 类 号：R285.5[医药卫生—中药学]