杀菌性/通透性增加蛋白模拟肽对内毒素/脂多糖致小鼠急性肺损伤的保护作用  

Protective effects of bactericidal/permeability increasing protein simulated peptide on murine acute lung injury induced by lipopolysaccharide

作　　者：高宏富[1] 袁建成[1] 肖光夏[1] 

机构地区：[1]第三军医大学西南医院全军烧伤研究所,创伤,烧伤与复合伤国家重点实验室,重庆400038

出　　处：《中华烧伤杂志》2005年第2期100-103,共4页Chinese Journal of Burns

基　　金：国家重点基础研究发展规划资助项目(G1999054203)

摘　　要：目的　观察杀菌性/通透性增加蛋白模拟肽(BNEP)对内毒素/脂多糖(LPS)致小鼠急性肺损伤(ALI)的作用。　方法　将BALB/c小鼠随机分为对照组、LPS组、BNEP组,每组20只。LPS组和BNEP组分别经鼻滴注等渗盐水(NS)+LPS和NS+LPS+尾静脉注射BNEP复制小鼠ALI模型。对照组处理方式类似,但仅滴注NS.检测各组小鼠肺脏湿干重比、肺血管通透性、肺组织病理学变化,应用免疫组织化学法检测肺组织中Toll样受体(TLR) 2、4表达水平的变化。　结果　BNEP组与LPS组比较,肺湿干重比减小,肺血管通透性降低,肺内以中性粒细胞为主的炎性细胞浸润减轻, TLR2、4在肺组织中的表达减弱(两组TLR2分别为128±10、214±12,P<0. 01).　结论　BNEP对由LPS引起的小鼠ALI有较好的保护作用。Objective To investigate the protective effects of bactericidal/permeability increasing protein simulated peptide (bactericidal neutralizing endotoxin protein, BNEP) on murine acute lung injury (ALI) induced by lipopolysaccharide (LPS). Methods A murine model of ALI was reproduced by lipopolysaccharide via intranasal instillation. The Balb/c mice were randomly divided into control (n=20, with nasal instillation of isotonic saline), LPS instillation (n=20, with nasal instillation of isotonic saline and LPS) and BNEP treatment (n=20, with nasal instillation of isotonic saline plus LPS and BNEP) groups. The ratio of lung wet weight to dry weight, the permeability of pulmonary capillary vessels and the histopathology of pulmonary tissue were determined in all groups. The change in the expression of Toll-like receptor 2 and 4 (TLR2/4) in the pulmonary tissue was detected by immunohistochemistry. Results Compared with LPS instillation group, the ratio of lung wet weight to dry weight and the permeability of pulmonary capillary vessel was decreased significantly in the BNEP group, and the inflammatory infiltration in the pulmonary tissue induced by neutrophil influx was alleviated markedly with BNEP treatment. The expression of TLR2 and TLR4 in pulmonary vascular endothelial cells, macrophages and alveolar type Ⅱ epithelial cells in BNEP group were lower than those in LPS group (TLR2:128±10 vs 214±12, P <0.01).Conclusion BNEP, as a simulated peptide of BPI, exerted a remarkable protective effect on ALI induced by LPS.[

关 键 词：急性肺损伤 蛋白模拟肽 保护作用 内毒素 杀菌性 脂多糖(LPS) BALB/c小鼠 肺血管通透性 Toll样受体 免疫组织化学法 BNEP 炎性细胞浸润 TLR2 尾静脉注射 病理学变化 中性粒细胞 肺组织 经鼻滴注 对照组 ALI 处理方 ALl 

分 类 号：R563[医药卫生—呼吸系统] R978.16[医药卫生—内科学]