深Ⅱ度烫伤大鼠创缘皮肤组织中表皮细胞增殖的研究  

Study on the proliferation of epidermal cells of wound edge in deep partial thickness scald injury in rat

作　　者：谢挺[1] 陆树良[1] 牛轶雯[1] 葛奎[1] 田鸣[1] 董炜[1] 青春[1] 史济湘[1] 

机构地区：[1]第二医科大学瑞金医院烧伤科,上海200025

出　　处：《中华烧伤杂志》2005年第2期128-131,共4页Chinese Journal of Burns

基　　金：国家重点基础研究发展规划资助项目(G1999054205)

摘　　要：目的　观察大鼠深Ⅱ度烫伤后创缘皮肤组织中表皮细胞的增殖活动规律,探讨其可能机制。　方法　将24只SD大鼠随机分为正常组(未烫伤)、烫伤后3d组、7d组及14d组,每组6只。采集正常组大鼠皮肤及烫伤大鼠创缘皮肤,观察其表皮组织学特征;用流式细胞仪检测表皮细胞的细胞周期;蛋白印迹法检测表皮细胞增殖调节因子细胞周期素(cyclinD1、cyclinB1)和细胞周期素依赖性激酶(CDK)4的表达水平,并测定M期促进因子(MPF)的组蛋白激酶活性。　结果　组织学观察显示,伤后3d组大鼠创缘表皮细胞核及核仁较正常组增大;伤后14d组胞核及核仁增大明显,细胞数显著增多。伤后14d组S期表皮细胞百分比出现上升趋势;G2 /M期百分比自伤后3d起逐渐升高,伤后7、14d组(4. 5±0. 6、5. 4±1. 0)显著高于正常组(2. 9±1. 1,P<0. 05).cyclinD1表达量伤后3d即明显增高;cyclinB1表达无显著波动。伤后3d组的CDK4表达水平较低, 14d组开始回升。伤后14d组表皮细胞MPF活性显著增强。　结论　在细胞周期调控因子作用下,大鼠深Ⅱ度烫伤后皮肤组织中表皮细胞在伤后早期即出现DNA合成及有丝分裂的增加,伤后14d增殖明显活跃。cyclinD1 /CDK4复合物的表达及MPF的活性在伤后早期并未同步增高,但自伤后14d起其表达显著增多,提示创面愈合过程中细胞周期调?Objective To investigate the rule and possible mechanism of epidermal proliferation in wound edge of deep partial thickness scald injury in rat. Methods Twenty-four Sprague-Dawley rats inflicted with deep partial thickness scald were randomized into pre-scalding, 3 post-scalding day (PSD), 7PSD and 14PSD groups, with 6 rats in each group. Skin specimens from the wound edge were harvested for the observation of the histological characteristics of the epidermis. Cell cycles of epidermal cells were analyzed with flow cytometry. The expressions of cyclin D1, cyclin B1, cdk4 and the histone H1 kinase activity of MPF in epidermal cells were determined by Western blotting. Results Augmentation of nuclei and nucleoli was found in the epidermal cells from the wound edge in 3PSD group, while increased number of epidermal cells with obviously augmented nuclei and nucleoli were found in 14PSD group. The percentage of the cells in S phase increased in 14 PSD group. The percentage of epidermal cells in G2/M phase began to increase in 3PSD group, and that in 7PSD (4.5±0.6)and 14PSD (5.4±1.0) groups were obviously higher than that in pre-scalding group (2.9±1.1, P <0.05). The expression of cyclin D1 increased significantly in 3PSD group. The expression of cdk4 decreased in 3PSD group, but began to increase in 14PSD group. There was no difference in the expression of cyclin B1 among groups. The MPF activity was significantly increased in 14PSD group. Conclusion There was enhanced DNA synthesis and mitosis in epidermal cells of rats with deep partial scald during early post-scald stage, and active proliferation of epidermal cells was observed on 14PSD. The expression of cyclinD1/cdk4 complex and the activity of MPF increased since 14PSD, indicating that there was a special regulative pattern during wound healing.[

关 键 词：表皮细胞增殖 皮肤组织 烫伤大鼠 cyclinD1 细胞周期素依赖性激酶 CYCLINB1 细胞周期调控 流式细胞仪检测 深Ⅱ度烫伤 蛋白激酶活性 M期促进因子 CDK4 组织学特征 蛋白印迹法 组织学观察 表皮细胞核 DNA合成 正常组 活动规律 

分 类 号：R644[医药卫生—外科学]