利什曼原虫—DNA重复序列在虫种鉴定上的价值分析  

Evaluation of a repetitive DNA sequence of Leishmania on the identification of Leishmania isolates in China

作　　者：汪俊云[1] 高春花[1] 杨玥涛[1] 包意芳[1] 

机构地区：[1]中国疾病预防控制中心寄生虫病预防控制所,上海200025

出　　处：《中国人兽共患病杂志》2005年第4期304-308,共5页Chinese Journal of Zoonoses

基　　金：世界卫生组织TSA基金资助项目 (NO .10 79946)

摘　　要：目的　通过分析和比较我国利什曼原虫分离株与相应的利什曼原虫世界卫生组织(WHO)参照株在一种DNA重复序列上的同源性以考察这一DNA重复序列应用于鉴别我国利什曼原虫的价值。方法　PCR扩增各利什曼原虫分离株的DNA重复序列片段并测序,用GENEDOC软件比较扩增的各分离株DNA重复序列的同源性。结果　该DNA重复序列在种内各利什曼原虫分离株间完全同源或高度同源(99%～10 0 % ) ,且碱基变异具有高度稳定性,而在种间则显示相当的差异(一般同源性小于90 % )。结论　该DNA重复序列在我国利什曼原虫的鉴定上有较好的实用价值。The homologies between the Leishmania isolates in China and the reference strain of WHO were analysed on the basis of determination of a repetitive sequence of Leishmania. The repetitive DNA fragments of the Leishmania isolates were amplified by PCR and their sequences were determined by using the PCR products. Homology of the amplified repetitive sequences of the isolated strains were then compared with GENEDOC software program.It was found that alignment of sequences showed a complete or higher homologies (99%～100%) among the different strains of species with a considerable difference between different species (usually less than 90%). These results prove the practical value of this repetitive DNA fragment on the identification of the Leishmania isolates in China.

关 键 词：利什曼原虫 DNA重复序列 虫株鉴定 

分 类 号：R382.2[医药卫生—医学寄生虫学]