蓝氏贾第虫病毒体外转录体转染条件的研究  

Study on the optimal transfection condition for the in vitro transcripts from Giardia lamblia virus

作　　者：刘全[1] 张西臣[1] 李建华[1] 尹继刚[1] 赵永军[1] 

机构地区：[1]解放军军需大学军事兽医系

出　　处：《中国人兽共患病杂志》2005年第4期329-330,334,共3页Chinese Journal of Zoonoses

基　　金：国家自然科学基金资助项目 (No .3 0 3 0 0 2 60 )

摘　　要：目的　确定蓝氏贾第虫病毒体外转录体电穿孔转染的最佳条件。方法　以不同的电击缓冲液、电压及脉冲时间应用GLV和GFP嵌合体体外转录体电穿孔转染蓝氏贾第虫,测定转染虫体的存活率及GFP表达量。结果　在不同转染条件下GLV和GFP嵌合体体外转录体均能成功转染蓝氏贾第虫,且在cytomix缓冲液、10 0 0V/cm、8ms电击条件下,对虫体损伤最小,GFP表达量最高。结论　本试验确定的蓝氏贾第虫病毒体外转录体电穿孔转染最佳条件是电击缓冲液为cytomix缓冲液,电压为10 0 0V/cm、脉冲时间为8ms。To determine the optimal transfection condition for the in vitro transcripts from Giardia lamblia virus (GLV), the transcript from chimera of GLV and the green fluorescent protein (GFP) was electroporated into Giardia lamblia in different buffers, voltages and impulse times. The survival rate of parasites was calculated, and the GFP expression was determined by ELISA. Experimental result showed that the transcript from chimera of GLV and GFP was successfully transfected into Giardia lamblia. The damages on the parasites was minimized and the expression of GFP was to be highest when the optimal conditions for transfection was established in cytomix buffer with the electroporation under 1000 V/cm and 8ms.

关 键 词：蓝氏贾第虫病毒 电穿孔 绿色荧光蛋白 ELISA 

分 类 号：S852.7[农业科学—基础兽医学]