利用RNA介导的抗病性获得抗2种病毒的转基因烟草  

Production of transgenic tobacco plants resistant to two viruses via RNA-mediated virus resistance

作　　者：白庆荣[1] 朱俊华[1] 刘晓玲[1] 朱常香[2] 宋云枝[2] 温孚江[1] 

机构地区：[1]山东农业大学植物保护学院,泰安271018 [2]山东农业大学生命科学学院,泰安271018

出　　处：《植物病理学报》2005年第2期148-154,共7页Acta Phytopathologica Sinica

基　　金：国家自然科学基金资助项目(30270875);山东省科学技术发展计划资助项目(022090131)

摘　　要：RNA介导的病毒抗性(RMVR)是近年发展起来的一种新的植物抗病毒基因工程策略,具有抗病性强、抗性持久、生物安全性高等特点。利用该策略培育多抗病毒植株具有广阔的应用前景和重要的实践意义。本研究将非翻译的马铃薯X病毒的衣壳蛋白(PVX-CP)基因和非翻译的马铃薯Y病毒的衣壳蛋白(PVY-CP)基因组成嵌合基因,构建植物表达载体pROKXY,利用农杆菌介导法转化烟草NC89,获得6株对PVX和PVY的混合侵染表现为免疫的转基因植株。分子分析表明,这种抗性为RNA介导的病毒抗性。这一研究结果为利用RMVR进行植物多抗病毒育种提供了重要数据和资料。RNA-mediated virus resistance is an effective way to obtain virus resistant plants. The strategy is provided with the advantages of high bio-safety and long resistant duration. The method is regarded as a potential strategy with broad application value and practical significance in plant genetic engineering against viruses. In this study,we used untranslatable CP genes of Potato virus X(PVX-CP) and Potato virus Y(PVY-CP) to construct a chimeric cDNA fragment,which was then introduced into the plant expressing vector pROKII. The recombinant binary vector pROKXY was introduced into tobacco (NC89) plants via Agrobacterium tumefaciens-mediated transformation,six transgenic plants immune to the co-infection with PVX and PVY Nwere obtained. Molecular analysis demonstrated that the resistance was RNA-mediated virus resistance.

关 键 词：RNA介导的病毒抗性 马铃薯X病毒 马铃薯Y病毒坏死株系 嵌合基因 转基因烟草 

分 类 号：Q943.2[生物学—植物学] S432.41[农业科学—植物病理学]