波形蛋白和S-100β在T1A系和T2A系细胞分化中的表达差异  

Expression difference of vimentin and S-100β in cultured T1A lineageand T2A lineage cells during their differentiation

作　　者：孙燕[1] 夏春林[2] 王劼[1] 何炎[2] 

机构地区：[1]苏州大学医学院人体解剖学教研室暨神经细胞生物学研究室,苏州215007 [2]复旦大学上海医学院解剖与组织胚胎学系

出　　处：《解剖学杂志》2005年第2期121-123,F002,共4页Chinese Journal of Anatomy

基　　金：苏州大学医学院发展基金(EF134004);复旦大学青年基金(EXF101369

摘　　要：目的:观察波形蛋白和S-100β在T1A和T2A系细胞分化中的表达差异。方法:根据T1A、O2A祖细胞的生长时间差异及细胞的黏附特性不同,采用振荡法和差速贴壁法,分别纯化T1A系细胞(T1A前体细胞和T1A)和T2A系细胞(O2A祖细胞、未成熟T2A和成熟T2A);免疫细胞化学法观察波形蛋白和S-100β在T1A系和T2A系细胞中的表达情况。结果:T1A前体细胞、T1A和O2A祖细胞均表达波形蛋白和S-100β;未成熟T2A只表达S-100β,成熟T2A不表达波形蛋白和S-100β。结论:在星形胶质细胞的发育过程中,波形蛋白和S-100β的表达可能存在时间和谱系间的差异。Objective:To observe the differential expression of vimentin and S-100β during the development of T1A lineage cells and T2A lineage cells.Methods:Based on the differential cellular adhesions and developmental time-course properties of T1A and O-2A progenitors, T1A lineage cells and T2A lineage cells were purified with a standard shaking method and the differential adhesion method. The expression of vimentin and S-100β in T1A lineage cells and T2A lineage cells were observed with immunocytochemical label. Results:T1A progenitors, T1A and O-2A progenitors expressed both vimentin and S-100β. The immature T2A only expressed S-100β, whereas the mature T2A did not express the 2 antigens. Conclusion:The expression of vimentin and S-100β exist differences in time and cell lineage during the development of astrocytes.

关 键 词：S-100Β 波形蛋白 表达差异 细胞分化 O-2A祖细胞 免疫细胞化学法 星形胶质细胞 前体细胞 时间差异 表达情况 发育过程 存在时间 未成熟 贴壁法 振荡法 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]