过量维生素A酸对金黄地鼠胚胎神经管维生素A酸受体α、β基因表达的影响  

Effects of excessive retinoic acid on expression of RARα and RARβgenes in neural tube of golden hamster embryos

作　　者：芦志红[1] 刘凯[1] 高英茂[1] 李少玲[1] 

机构地区：[1]山东大学医学院组织学与胚胎学教研室,济南250012

出　　处：《解剖学杂志》2005年第2期157-160,共4页Chinese Journal of Anatomy

基　　金：山东省自然科学基金(Y99C01)

摘　　要：目的:探讨过量维生素A酸(RA)对金黄地鼠胚胎神经管RA受体α和RA受体β基因表达的影响。方法:采用RTPCR方法,半定量分析金黄地鼠神经管正常发生及RA致神经管畸形过程中RA受体α和RA受体βmRNA表达水平的变化。结果:过量RA可使RA受体α和RA受体βmRNA在金黄地鼠神经管中的表达水平呈现短时下降,而后又大幅上升的变化。结论:过量RA引起的RA受体α和RA受体βmRNA表达水平的变化与RA致金黄地鼠神经管畸形相关。Objective: To investigate the effect of excessive retinoic acid (RA) on the expression of RARα and RARβ in the neural tube of golden hamster embryos. Methods: The changes of the RARα mRNA and RARβ mRNA induced by excessive RA in normally developed neural tube and neural tube defects of golden hamsters were analyzed with RT-PCR. Results: Excessive RA down-regulated the expressions of RARα mRNA and RARβ mRNA in the neural tube of golden hamster initially, and then up-regulated them significantly. Conclusion:The changes of RARα mRNA and RARβ mRNA expression induced by excessive RA are correlated with the neural tube defects of golden hamster induced by RA.

关 键 词：维生素A酸 基因表达 受体Α 鼠胚胎 过量 RT-PCR方法 mRNA表达 神经管畸形 金黄地鼠 半定量分析 A受体 RA 水平 

分 类 号：R715[医药卫生—妇产科学]