左旋18-甲基炔诺酮对大鼠下丘脑GnRH神经元的影响  

Effect of levonorgestrel on gonadotropin-releasing hormone positive neurons in rat hypothalamus

作　　者：崔慧先[1] 刘晓云[2] 康林[1] 赵春芳[3] 

机构地区：[1]河北医科大学解剖学教研室,石家庄050017 [2]河北医科大学第二医院神经内科,石家庄050017 [3]河北医科大学组织学与胚胎学教研室,石家庄050017

出　　处：《解剖学杂志》2005年第2期182-184,i004,共4页Chinese Journal of Anatomy

摘　　要：目的:观察孕激素类避孕药—左旋18-甲基炔诺酮(LNG)对大鼠下丘脑GnRH神经元的影响。方法:正常雌性大鼠分为长期给药组(灌服LNG90d)和停药组(停药后正常喂养20d)。免疫组织化学方法显示下丘脑GnRH阳性神经元。结果:长期给药组与对照组相比斜角带(db)、视前区(MPA)内GnRH阳性神经元构成比发生改变,棘型神经元减少,阳性神经元光密度减低,阳性纤维膨体密度降低,正中隆起(ME)处阳性纤维密度增加。停药后基本恢复正常。结论:从形态学上证实LNG作用于下丘脑水平;停药后GnRH阳性神经元形态学变化可恢复正常,表明LNG具有高效安全性。Objective:To study the changes of gonadotropin-releasing hormone (GnRH) positive neurons in levonorgestrel (LNG) treatment rat hypothalamus. Methods: Female Sprague-Dawlay rats were divided into long-term treatment (LNG 90 d) group and drug-stop treatment group (20 d normal feeding after given drug). The changes of gonadotropin-releasing hormone (GnRH) in hypothalamic were observed by immunocytochemistry. Results: Compared with control, cell composition and the quantity of GnRH positive fibers decreased in the long-term treatment group, but the terminals in median eminence (ME) contained significantly more GnRH. In the drug-stop treatment group, no difference was found as compared with control group. The changes recovered to the normal level after stopping drug.Conclusion: The results demonstrate that oral contraceptives exert their effect primarily at the hypothalamic level. All changes can return to the normal after ceasing treatment, so it can be concluded that LNG is a kind of oral contraceptive with high effect and safe quality.

关 键 词：左旋18-甲基炔诺酮 GnRH 下丘脑 阳性神经元 免疫组织化学方法 激素类避孕药 形态学变化 停药后 雌性大鼠 阳性纤维 纤维密度 正中隆起 LNG 药组 斜角带 对照组 视前区 光密度 体密度 安全性 恢复 

分 类 号：R96[医药卫生—药理学]